A New Coral Carbonic Anhydrase in Stylophora pistillata
A New Coral Carbonic Anhydrase in Stylophora pistillata
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DOI：
10.1007/s10126-011-9363-x
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发表时间：
2011-10-01
影响因子：
3
通讯作者：
Zoccola, Didier
中科院分区：
文献类型：
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作者：
Bertucci, Anthony;Tambutte, Sylvie;Zoccola, Didier
硬珊瑚是特别感兴趣的，因为它们能够建立一个细胞内的互利共生与光养甲藻和存款高利率的碳酸钙在他们的骨架。碳酸酐酶已被证明在这两个过程中起着至关重要的作用。在这项研究中，我们报告了一个新的α-CA的珊瑚Stylophora pistillata的分子克隆和表征。这种酶与人同种型CA II具有同源性，被称为STPCA-2。STPCA-2为35.2 kDa，具有催化活性的所有关键氨基酸。催化常数与米氏常数之比（k（cat）/K-m）为8.3.10（7）M-1 s（-1），被认为是高活性的。由于其在口腔内胚层和离口组织中的细胞内定位，我们提出STPCA-2参与pH调节和/或无机碳递送到共生体和钙化。
Scleractinian corals are of particular interest due to their ability to establish an intracellular mutualistic symbiosis with phototrophic dinoflagellates and to deposit high rates of calcium carbonate in their skeleton. Carbonic anhydrases have been shown to play a crucial role in both processes. In this study, we report the molecular cloning and characterization of a novel alpha-CA in the coral Stylophora pistillata. This enzyme shares homologies with the human isoform CA II and is referred to as STPCA-2. STPCA-2 is 35.2 kDa and possesses all key amino acids for catalytic activity. With a ratio between catalytic and Michaelis constants (k(cat)/K-m) of 8.3.10(7) M-1 s(-1) is considered as highly active. Owing to its intracellular localisation in the oral endoderm and in the aboral tissue, we propose that STPCA-2 is involved in pH regulation and/or inorganic carbon delivery to symbiont and calcification.