Model to quantify lymph node enhancement on indirect sonographic lymphography
Model to quantify lymph node enhancement on indirect sonographic lymphography
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DOI：
10.2214/ajr.183.2.1830513
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发表时间：
2004-08-01
影响因子：
5
通讯作者：
Mattrey, RF
中科院分区：
文献类型：
--
作者：
Choi, SH;Kono, Y;Mattrey, RF
OBJECTIVE.我们的目标是开发一种可靠的技术，具有最小的操作员依赖性量化淋巴结增强，以测试和优化新的超声造影剂配方。使用标记为A-G的五种试剂对20只健康家兔进行了研究。试剂D和E是相同的试剂，试剂F和G是Imagent，盲法研究以测试再现性。将1毫升造影剂注入每个后足垫。将13 MHz换能器固定在腘淋巴结上，使用相位反转技术在4.8 MHz中心发射频率下以100%功率和每秒一帧成像。每次注射后，立即按摩足垫12次，每次30秒，然后在每次按摩后成像，以评估每次注射后淋巴结可以重新填充的次数。测量淋巴结视频强度，并使用方差分析评估增强程度，其中按摩次数和药剂用作自变量。所有药物在第一次按摩后均观察到淋巴结增强。A和B剂的增强程度最小，D和F剂居中，C剂最大。A剂在前两次按摩后有效，B剂在前四次按摩后有效，C剂在所有12次按摩后有效，D剂在前八次按摩后有效，F剂在前九次按摩后有效。药剂D和F的性能与它们的复制品E和G相似。我们建立了一种可重复的技术来量化淋巴结增强，可以区分不同的因子。性能的差异表明，有可能优化制剂的间接超声淋巴造影。
OBJECTIVE. Our goal was to develop a reliable technique that has minimal operator dependence for quantifying lymph node enhancement to test and optimize new sonography contrast formulations.MATERIALS AND METHODS. Twenty healthy rabbits were studied using five agents, labeled A-G. Agents D and E were the same agent and agents F and G were Imagent, studied blindly to test reproducibility. One milliliter of contrast agent was injected into each hind footpad. A 13-MHz transducer was fixed over the popliteal node, which was imaged at a 4.8-MHz central transmit frequency using phase-inversion technology at 100% power and one frame per second. Immediately after each injection, the footpad was massaged 12 times for 30 sec each time and then imaged after each massage to assess the number of times the node could be refilled from each injection. Lymph node video intensity was measured, and the degree of enhancement was evaluated using analysis of variance with the massage number and the agent used as independent variables.RESULTS. Lymph node enhancement was observed after the first massage with all agents. Degree of enhancement was least with agents A and B, intermediate with agents D and F, and greatest with agent C. Agent A was effective after the first two massages, agent B after the first four, agent C after all 12, agent D after the first eight, and agent F after the first nine. Performance of agents D and F was similar to that of their duplicates, E and G.CONCLUSION. We established a reproducible technique to quantify lymph node enhancement that can distinguish between different agents. The differences in performance suggest that it is possible to optimize agent formulation for indirect sonographic lymphography.