The levels of macrophage migration inhibitory factor as an indicator of disease activity and severity in adult-onset Still's disease.
The levels of macrophage migration inhibitory factor as an indicator of disease activity and severity in adult-onset Still's disease.
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DOI：
10.1016/j.clinbiochem.2008.01.008
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发表时间：
2008-05
影响因子：
2.8
通讯作者：
Yu-Qiong Zou;L. Lu;Shu-jie Li;Ting Zeng;Xiao-dong Wang;C. Bao;Shun‐le Chen;Cheng-de Yang
中科院分区：
文献类型：
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作者：
Yu-Qiong Zou;L. Lu;Shu-jie Li;Ting Zeng;Xiao-dong Wang;C. Bao;Shun‐le Chen;Cheng-de Yang
本研究探讨了巨噬细胞移动抑制因子（MIF）在成人型斯蒂尔病（AOSD）的水平，并探讨了这种促炎细胞因子在全身炎症AOSD.DESIGN和METHODS的作用血清MIF水平测定与AOSD患者和对照组。三色流式细胞仪检测外周血白细胞产生MIF的能力。结果表明：AOSD患者血清MIF水平明显升高。血清MIF水平在伴有咽喉痛、肌痛、脾肿大或胸膜炎的AOSD患者中显著升高，并且与临床疾病的严重程度和活动性密切相关。通过流式细胞仪检测，从AOSD患者的单核细胞和T-淋巴细胞的细胞内MIF水平显着高于那些从健康subjects. CONCLUSION这些数据代表的第一个演示增加的MIF表达在AOSD，并建议，MIF可能是一个重要的标志物，疾病的评估和监测。
OBJECTIVESThis study investigated the levels of macrophage migration inhibitory factor (MIF) in adult-onset Still's disease (AOSD) and explored the role of this pro-inflammatory cytokine in the systemic inflammation of AOSD.DESIGN AND METHODSSerum MIF levels were measured by ELISA in patients with AOSD and controls. Intracellular MIF production by peripheral blood leukocytes was detected by three-color flow cytometry.RESULTSSerum MIF levels were significantly increased in patients with AOSD. Serum MIF levels were significantly higher in AOSD patients with sore throat, myalgias, splenomegaly, or pleuritis, and were closely correlated with clinical disease severity and activity. Examined by flow cytometry, the intracellular MIF levels in monocytes and T-lymphocytes from AOSD patients were significantly higher than those from healthy subjects.CONCLUSIONThese data represent the first demonstration of increased MIF expression in AOSD, and suggest that MIF may be an important marker for disease evaluation and monitoring.