Characterization of microRNA expression in serous ovarian carcinoma
Characterization of microRNA expression in serous ovarian carcinoma
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DOI：
10.3892/ijmm.2014.1813
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发表时间：
2014-08-01
影响因子：
5.4
通讯作者：
Zhang, Wei
中科院分区：
文献类型：
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作者：
Li, Yanhong;Yao, Li;Zhang, Wei
浆液性卵巢癌是一种常见的妇科恶性肿瘤，5年生存率低。然而，关于卵巢肿瘤发生的行为和遗传学知之甚少。MicroRNAs（miRNAs）在卵巢癌中表达异常。为了评估miRNA在浆液性卵巢癌中的表达谱，我们使用miRNA微阵列确定了100个福尔马林固定、石蜡包埋的卵巢癌组织块以及50个相应的正常输卵管组织中的miRNA表达模式。与对照组相比，浆液性卵巢癌组织中有63个miRNAs表达下调，43个miRNAs表达上调。使用定量聚合酶链反应（RT-qPCR）验证五种失调的miRNA的表达。GO术语和通路分析显示，细胞周期的生物学过程显著富集，MAPK信号通路高度参与卵巢癌的进展。结果提示，miRNAs的异常表达参与了卵巢癌的发生，因此这些miRNAs可能作为诊断和预后的生物标志物。
Serous ovarian cancer is a major gynecologic malignancy with a poor 5-year survival rate. However, little is known regarding the behavior and genetics of ovarian tumorigenesis. MicroRNAs (miRNAs) have been shown to be dysregulated in ovarian carcinomas. To assess the miRNA expression profiles in serous ovarian cancer, we defined the patterns of miRNA expression in 100 formalin-fixed, paraffin-embedded ovarian cancer tissues blocks as well as 50 corresponding normal oviduct tissues using miRNA microarray. MiRNA expression profiling showed that 63 miRNAs were downregulated and 43 miRNAs were upregulated in serous ovarian cancer tissues compared with control tissues. The expression of five dysregulated miRNAs was validated using quantitative polymerase chain reaction (RT-qPCR). GO term and pathway analysis revealed that the biological process of the cell cycle was significantly enriched and the MAPK signaling pathway was highly involved in the progression of ovarian cancer. The results suggested that the aberrant expression of miRNAs is involved in ovarian carcinogenesis and thus these miRNAs may function as diagnostic and prognostic biomarkers.