Uterine Natural killer cells severely decrease in Number at gestation day 6 in mice
Uterine Natural killer cells severely decrease in Number at gestation day 6 in mice
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小鼠妊娠第 6 天子宫自然杀伤细胞数量严重减少
DOI：
10.1095/biolreprod.113.109009
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发表时间：
2013
影响因子：
3.6
通讯作者：
Terutaka Kakiuchi
中科院分区：
文献类型：
--
作者：
Akiko Takashima;Fumio Ishikawa;Taku Kuwabara;Yuriko Tanaka;Toshihiko Kinoshita;Motohiro Ito;Terutaka Kakiuchi
子宫自然杀伤（uNK）细胞在着床后数量显著增加。NK细胞或其前体细胞从血流中迁移并有助于增加。然而，处女子宫中存在的uNK细胞的贡献尚不清楚。为了阐明这一问题，我们检查了子宫白细胞亚群在怀孕期间的BALB/c小鼠。最显著的变化是妊娠第6天（gd）CD 11b −或Gr-1−细胞的大量减少。妊娠第0天的子宫NK细胞为CD 11b −，并在妊娠第6天严重降低。这种减少是选择性的，检查的其他细胞的比例没有减少。子宫NK细胞在gd 12时几乎恢复。这些细胞在gd 12比gd 0更成熟和/或活化，表达CD 11b、CD 27、CD 127或B220。在妊娠第0天或第6天在子宫细胞上观察到CXCL 12表达，但在妊娠第12天未观察到，而在妊娠第0天和第12天在uNK细胞上检测到CXCR 4。在gd 12观察到子宫细胞中IL-15的表达或uNK细胞中干扰素-γ的表达比gd 0高得多。在gd 12时在uNK细胞上检测到IL-15受体α链，但在gd 0时未检测到。总之，这些发现与我们的解释一致，即gd 0时存在的uNK细胞不会导致着床后uNK细胞数量增加，NK细胞或其前体细胞迁移到子宫内，成熟并产生干扰素-γ以支持妊娠。
Uterine natural killer (uNK) cells remarkably increase in number after implantation. NK cells or their precursors migrate from the blood stream and contribute to the increase. However, the contribution of uNK cells present in the virgin uterus has been unclear. To elucidate this issue, we examined uterine leukocyte subsets during pregnancy in BALB/c mice. The most dramatic change was the massive decrease in CD11b−or Gr-1−cells at Gestation Day (gd) 6. Uterine NK cells at gd 0 were CD11b−, and severely decreased at gd 6. The decrease was selective, and the proportion of other cells examined did not decrease. Uterine NK cells almost recovered at gd 12. These cells at gd 12 were more mature and/or activated in terms of expression of CD11b, CD27, CD127, or B220 than at gd 0. CXCL12 expression was observed on uterine cells at gd 0 or 6, but not at gd 12, whereas CXCR4 was detected on uNK cells at gds 0 and 12. A much higher expression of IL-15 in uterine cells or interferon-gamma expression in uNK cells was observed at gd 12 than at gd 0. IL-15 receptor alpha chain was detected on uNK cells at gd 12, but not at gd 0. Taken together, these findings were consistent with our interpretation that uNK cells present at gd 0 do not contribute to the increase of uNK cell number after implantation, and NK cells or their precursors migrate into the uterus, mature, and produce interferon-gamma to support pregnancy.