Limited T cell receptor repertoire diversity in tuberculosis patients correlates with clinical severity.
Limited T cell receptor repertoire diversity in tuberculosis patients correlates with clinical severity.
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DOI：
10.1371/journal.pone.0048117
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ma L
中科院分区：
文献类型：
--
作者：
Luo W;Su J;Zhang XB;Yang Z;Zhou MQ;Jiang ZM;Hao PP;Liu SD;Wen Q;Jin Q;Ma L
The importance of CD4+ and CD8+ T cells in protection against tuberculosis (TB) is well known, however, the association between changes to the T cell repertoire and disease presentation has never been analyzed. Characterization of T-cells in TB patients in previous study only analyzed the TCR β chain and omitted analysis of the Vα family even though α chain also contribute to antigen recognition. Furthermore, limited information is available regarding the heterogeneity compartment and overall function of the T cells in TB patients as well as the common TCR structural features of Mtb antigen specific T cells among the vast numbers of TB patients. CDR3 spectratypes of CD4+ and CD8+ T cells were analyzed from 86 patients with TB exhibiting differing degrees of disease severity, and CDR3 spectratype complexity scoring system was used to characterize TCR repertoire diversity. TB patients with history of other chronic disease and other bacterial or viral infections were excluded for the study to decrease the likely contribution of TCRs specific to non-TB antigens as far as possible. Each patient was age-matched with a healthy donor group to control for age variability. Results showed that healthy controls had a normally diversified TCR repertoire while TB patients represented with restricted TCR repertoire. Patients with mild disease had the highest diversity of TCR repertoire while severely infected patients had the lowest, which suggest TCR repertoire diversity inversely correlates with disease severity. In addition, TB patients showed preferred usage of certain TCR types and have a bias in the usage of variable (V) and joining (J) gene segments and N nucleotide insertions. Results from this study promote a better knowledge about the public characteristics of T cells among TB patients and provides new insight into the TCR repertoire associated with clinic presentation in TB patients.
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影响因子：
3.7
作者：
Caccamo N;Guggino G;Meraviglia S;Gelsomino G;Di Carlo P;Titone L;Bocchino M;Galati D;Matarese A;Nouta J;Klein MR;Salerno A;Sanduzzi A;Dieli F;Ottenhoff TH
通讯作者：
Ottenhoff TH
影响因子：
4.4
作者：
Sud, Dhruv;Bigbee, Carolyn;Kirschner, Denise E.
通讯作者：
Kirschner, Denise E.
影响因子：
3.1
作者：
Andersson, Jan;Samarina, Arina;Grundstroem, Susanna
通讯作者：
Grundstroem, Susanna
影响因子：
1.5
作者：
Pahwa, S;Chitnis, V;Douglas, SD
通讯作者：
Douglas, SD
T细胞亚群在小鼠中的肺结核感染的免疫和免疫病理学中的相对重要性。
影响因子：
15.3
作者：
Mogues, T;Goodrich, M E;Ryan, L;LaCourse, R;North, R J
通讯作者：
North, R J