Adhesion of human osteoblasts on root-end filling materials
Adhesion of human osteoblasts on root-end filling materials
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DOI：
10.1097/00004770-200007000-00006
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发表时间：
2000-07-01
影响因子：
4.2
通讯作者：
Spangberg, LSW
中科院分区：
文献类型：
--
作者：
Zhu, Q;Haglund, R;Spangberg, LSW
扫描电镜观察人成骨细胞与根端填充材料（矿物三氧化骨料（MTA）、IRM、复合材料、银汞合金）的粘附情况，将根端填充材料插入96孔平底板中，压缩成与孔直径相近、厚度为1mm的圆盘。培养皿设置好后，放置于Nunc四孔培养板底部，每孔一个培养皿。将人成骨细胞按每孔1.5 × 10(5)个细胞的比例植入孔中，培养1 d后，用扫描电镜观察根端填充材料盘及其表面生长的细胞，结果显示成骨细胞在MTA和复合材料上呈单层附着、扩散，在汞齐合金上也有附着，但扩散的细胞很少。IRM存在时，成骨细胞呈圆形，无扩散。这些结果表明，与IRM和汞合金相比，MTA和复合树脂对成骨细胞有更好的反应。
Adhesion of human osteoblasts to root-end filling materials (mineral trioxide aggregate (MTA), IRM, composite, and amalgam) was observed by scanning electron microscopy, Root-end filling materials were inserted into 96-well flat-bottomed plates and condensed to disks of similar to 1 mm thick and the same diameter as the wells. After the disks were set, they were placed in the bottom of Nunc four-well culture plates at one disk per well. Then human osteoblasts were seeded into the wells at 1.5 x 10(5) cells per well, After 1 day in culture the disks of root-end filling materials along with cells grown on their surface were examined with a scanning electron microscopy, Results showed that osteoblasts attached and spread on MTA and composite by forming a monolayer, Osteoblasts also attached on amalgam, but with few cells spreading. In the presence of IRM, osteoblasts appeared rounded with no spreading. These results indicate that osteoblasts have a favorable response to MTA and composite resin compared with IRM and amalgam.