Reverse-phase high-performance liquid chromatography (HPLC) analysis of retinol and retinyl esters in mouse serum and tissues.
Reverse-phase high-performance liquid chromatography (HPLC) analysis of retinol and retinyl esters in mouse serum and tissues.
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DOI：
10.1007/978-1-60327-325-1_15
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发表时间：
2010
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能够测量生物样品中维甲酸(维生素A及其衍生物)的内源性代谢物是了解维生素A的重要生理作用的关键。多年来，已经发展了许多分析生物样品中不同维甲酸的分析方法和方法。对于这些化合物的常规分析来说，高效液相色谱法是最好的分析技术。然而，由于不同的维甲酸代谢物的化学性质不同，不同的维甲酸代谢物不能在一次色谱运行中准确地分离和定量。在这里，我们将描述一种反相高效液相等规法，该方法可以高准确度、高灵敏度和高可靠性地提取、分离、鉴定和定量全反式视黄醇和不同分子种类的视黄酸酯。
The ability to measure endogenous metabolites of retinoids (vitamin A and its derivatives) in biological samples is key to understanding the crucial physiological actions of vitamin A. Over the years, many assays and methods have been developed to analyze different retinoids in biological samples. Liquid chromatography is the best analytical technique for routine analysis of these compounds. However, due to their different chemical properties, different retinoid metabolites cannot be accurately separated and quantified in a single chromatographic run. Here, we will describe a reverse-phase HPLC isocratic method that enables extraction, separation, identification, and quantification of all-trans-retinol and different molecular species of retinyl ester with high accuracy, sensitivity, and reliability.