Analysis of flux estimates based on 13C-labelling experiments
Analysis of flux estimates based on 13C-labelling experiments
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DOI：
10.1046/j.1432-1033.2002.02959.x
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发表时间：
2002-06-01
期刊：
影响因子：
--
通讯作者：
Nielsen, J
中科院分区：
文献类型：
--
作者：
Christensen, B;Gombert, AK;Nielsen, J
Modelling of the fluxes in central metabolism can be performed by combining labelling experiments with metabolite balancing. Using this approach, multiple samples from a cultivation of Saccharomyces cerevisiae in metabolic and isotopic steady state were analysed, and the metabolic fluxes in central metabolism were estimated. In the various samples, the estimates of the central metabolic pathways, the tricarboxylic acid cycle, the oxidative pentose phosphate pathway and the anaplerotic pathway, showed an unprecedented reproducibility. The high reproducibility was obtained with fractional labellings of individual carbon atoms as the calculational base, illustrating that the more complex modelling using isotopomers is not necessarily superior with respect to reproducibility of the flux estimates. Based on these results some general difficulties in flux estimation are discussed.
