The bio-response of osteocytes and its regulation on osteoblasts under vibration
The bio-response of osteocytes and its regulation on osteoblasts under vibration
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振动下骨细胞的生物反应及其对成骨细胞的调控
DOI：
10.1002/cbin.10575
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发表时间：
2016
影响因子：
3.9
通讯作者：
Fan Yu-Bo
中科院分区：
文献类型：
--
作者：
Wu Xin-Tong;Sun Lian-Wen;Qi Hong-Yu;Shi Hao;Fan Yu-Bo
振动，特别是在低幅度和高频率（LMHF），已被证明是合成代谢的骨，但如何LMHF振动信号被骨细胞感知没有得到充分的研究。另一方面，骨细胞在剪切应力作用下的力学信号转导一直是科学家们关注的焦点。由于低幅度振动引起的应变很小，因此之前对剪应力的解释是否仍然适用于LMHF振动尚不清楚。本文基于骨细胞的真实的几何形状，建立了骨细胞的有限元模型，比较了骨细胞在低磁高频振动和剪切应力作用下的力学行为。研究了骨细胞对不同频率振动的生物反应，包括可溶性因子的分泌和细胞内钙离子浓度。研究了振动骨细胞条件培养液对成骨细胞的调节作用。有限元分析结果表明，LMHF振动下的细胞膜变形很小（峰值为1.09%）相比，剪切应力引起的变形（峰值为6.65%）。LMHF振动后，骨细胞的F-actin应力纤维发生重组，尤其是在核周围。30 Hz的振动对骨细胞和成骨细胞的成骨有促进作用，而90 Hz的振动则有抑制作用。这些结果得出结论，骨细胞对LMHF振动的生物反应是频率依赖性的，并且与核周边的细胞骨架而不是膜变形更相关。
Vibration, especially at low magnitude and high frequency (LMHF), was demonstrated to be anabolic for bone, but how the LMHF vibration signal is perceived by osteocytes is not fully studied. On the other hand, the mechanotransduction of osteocytes under shear stress has been scientists' primary focus for years. Due to the small strain caused by low‐magnitude vibration, whether the previous explanation for shear stress will still work for LMHF vibration is unknown. In this study, a finite element method (FEM) model based on the real geometrical shape of an osteocyte was built to compare the mechanical behaviors of osteocytes under LMHF vibration and shear stress. The bio‐response of osteocytes to vibration under different frequencies, including the secretion of soluble factors and the concentration of intracellular calcium, were studied. The regulating effect of the conditioned medium (CM) from vibrated osteocytes on osteoblasts was also studied. The FEM analysis result showed the cell membrane deformation under LMHF vibration was very small (with a peak value of 1.09%) as compared to the deformation caused by shear stress (with a peak value of 6.65%). The F‐actin stress fibers of osteocytes were reorganized, especially on the nucleus periphery after LMHF vibration. The vibration at 30 Hz has a promoting effect on osteocytes and the osteogenesis of osteoblasts, whereas vibration at 90 Hz was suppressive. These results lead to a conclusion that the bio‐response of osteocytes to LMHF vibration is frequency‐dependent and is more related to the cytoskeleton on nuclear periphery rather than the membrane deformation.