PEA3 activates CXCR4 transcription in MDA-MB-231 and MCF7 breast cancer cells
PEA3 activates CXCR4 transcription in MDA-MB-231 and MCF7 breast cancer cells
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PEA3 激活 MDA-MB-231 和 MCF7 乳腺癌细胞中的 CXCR4 转录
DOI：
10.1093/abbs/gmr070
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发表时间：
2011-10-01
影响因子：
3.7
通讯作者：
Wu, Jiong
中科院分区：
文献类型：
--
作者：
Gu, Shengmei;Chen, Li;Wu, Jiong
CXC趋化因子受体4 （CXCR4）是一种细胞表面受体，已被证明介导许多实体肿瘤的转移，包括肺、乳腺、肾脏和前列腺肿瘤。在本研究中，我们发现过表达ets变异基因4 （PEA3）可以提高人MDA-MB-231和MCF-7乳腺癌细胞中CXCR4 mRNA水平和CXCR4启动子活性。PEA3促进CXCR4表达和乳腺癌转移。染色质免疫沉淀实验表明，PEA3在转染PEA3表达载体的细胞中可以与CXCR4启动子结合。在MDA-MB-231和MCF-7细胞中，PEA3 siRNA降低了CXCR4启动子活性和PEA3与CXCR4启动子的结合。这些结果表明PEA3可以激活CXCR4启动子转录，促进乳腺癌转移。
CXC chemokine receptor 4 (CXCR4) is a cell surface receptor that has been shown to mediate the metastasis of many solid tumors including lung, breast, kidney, and prostate tumors. In this study, we found that overexpression of ets variant gene 4 (PEA3) could elevate CXCR4 mRNA level and CXCR4 promoter activity in human MDA-MB-231 and MCF-7 breast cancer cells. PEA3 promoted CXCR4 expression and breast cancer metastasis. Chromatin immunoprecipitation assay demonstrated that PEA3 could bind to the CXCR4 promoter in the cells transfected with PEA3 expression vector. PEA3 siRNA attenuated CXCR4 promoter activity and the binding of PEA3 to the CXCR4 promoter in MDA-MB-231 and MCF-7 cells. These results indicated that PEA3 could activate CXCR4 promoter transcription and promote breast cancer metastasis.