Persistent infiltration of CD45RC(-) CD4(+) T cells, Th2-like effector cells, in prolonging hepatic allografts in rats pretreated with a donor-specific blood transfusion
Persistent infiltration of CD45RC(-) CD4(+) T cells, Th2-like effector cells, in prolonging hepatic allografts in rats pretreated with a donor-specific blood transfusion
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DOI：
10.1002/hep.510250436
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发表时间：
1997-04-01
期刊：
影响因子：
13.5
通讯作者：
Ogawa, M
中科院分区：
文献类型：
--
作者：
Miyanari, N;Yamaguchi, Y;Ogawa, M
A single intravenous injection of freshly heparinized blood from a donor-specific blood transfusion (DST) seven days before transplantation significantly prolongs the subsequent survival of hepatic allografts from ACI(RT1(a)) to LEW(RT1(1)) rats. We used W3/25 (anti-CD4) and OX22 (anti-CD45RC: an isoform of leukocyte-common antigen [CD45R]) monoclonal antibodies to investigate the cellular identity of hepatic allograft infiltrates following transplantation. The number of CD4(+) and CD45RC(+) cells in untreated allografts increased equally over time by day seven. However, in DST-treated hepatic allografts, CD4(+) and CD45RC(+) cells also increased over time by day 14, but the increment in the number of CD4(+) cells was significantly greater than that in CD45RC(+) cells. While the number of CD4(+) cells remained persistently elevated in the hepatic allografts of rats pretreated with DST, they did not initiate rejection. Fluorescence-activated cell sorter (FACS) analysis revealed that the accumulated CD4(+) T cells could be divided into two subsets, CD45RC(-)CD4(+) and CD45RC(+)CD4(+) T cells, and that the ratio of CD45RC(-)CD4(+)/CD45RC(+)CD4(+) T cells in the hepatic allografts of recipients pretreated with DST was significantly greater than that in untreated allografts. Reverse-transcriptase polymerase chain reaction (RT-PCR) analysis demonstrated that CD45RC(-)CD4(+) T cells expressed interleukin (IL)-4 and IL-10 messenger RNA (mRNA), but not IL-2 and interferon gamma (IFN-gamma). The pattern of messenger RNA expression in hepatic allograft infiltrates from animals pretreated with DST provides compelling evidence for the selective in vivo preservation of T-helper (Th2)-specific cytokines in the rat system. Our studies show that CD45RC leukocyte-common antigen expression can define different populations of hepatic infiltrating CD4(+) T cells, A persistent infiltration of CD45RC(-)CD4(+) T cells, Th2-like effector cells, is characteristic of hepatic allo grafts with a prolonged survival in DST-pretreated rats.
