Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes.
Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes.
复制标题
非洲爪蟾卵母细胞中功能性 U1 和 U2 人两栖动物杂交 snRNP 的组装。
DOI：
10.1126/science.2970672
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Prives,C
中科院分区：
文献类型：
--
作者：
Pan,ZQ;Prives,C
当将与U1和U2小核RNA（snRNA）区域互补的寡核苷酸注射到非洲爪蟾细胞中时，分别迅速诱导U1和U2 snRNA的特异性降解，然后自身被降解。经过这样的处理，从显微注射的病毒DNA转录而来的猿猴病毒40（SV40）晚期前体mRNA的剪接在卵母细胞中被阻断。如果在将 SV40 DNA 引入卵母细胞之前，注射 HeLa 细胞 U1 或 U2 snRNA 并使其组装成小核核糖核蛋白颗粒 (snRNP) 样复合物，则 SV40 晚期 RNA 的剪接效率与未接受 U1 或 U2 寡核苷酸的卵母细胞中的一样有效。这表明卵母细胞可以形成由人类 snRNA 和两栖动物蛋白组成的功能齐全的杂交 U1 和 U2 snRNP。
Oligonucleotides complementary to regions of U1 and U2 small nuclear RNAs (snRNAs), when injected intoXenopus laevisoocytes, rapidly induced the specific degradation of U1 and U2 snRNAs, respectively, and then themselves were degraded. After such treatment, splicing of simian virus 40 (SV40) late pre-mRNA transcribed from microinjected viral DNA was blocked in oocytes. If before introduction of SV40 DNA into oocytes HeLa cell U1 or U2 snRNAs were injected and allowed to assemble into small nuclear ribonucleoprotein particle (snRNP)-like complexes, SV40 late RNA was as efficiently spliced as in oocytes that did not receive U1 or U2 oligonucleotides. This demonstrates that oocytes can form fully functional hybrid U1 and U2 snRNPs consisting of human snRNA and amphibian proteins.