In vitro infection of cells of the monocytic/macrophage lineage with bovine leukaemia virus
In vitro infection of cells of the monocytic/macrophage lineage with bovine leukaemia virus
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DOI：
10.1099/0022-1317-81-1-109
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发表时间：
2000-01-01
影响因子：
3.8
通讯作者：
Gómez-Lucía, E
中科院分区：
文献类型：
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作者：
Doménech, A;Goyache, J;Gómez-Lucía, E
致癌逆转录病毒牛白血病病毒（BLV）主要感染B细胞。大多数受感染的动物在可观察到循环B细胞或局部肿瘤增加之前长时间保持无症状。这种长的临床潜伏期可以通过单核细胞/巨噬细胞谱系（M/M）的细胞被感染并作为病毒的储存库来解释，如对于其他逆转录病毒（人免疫缺陷病毒-1、猫免疫缺陷病毒）所示。将不同分化阶段的M/M细胞（HL-60、THP-1、U-937、J774、BGM、PM 2、绵羊和奶牛的原代巨噬细胞）与永久感染供体细胞（FLKBLV和BLV-bat（2））产生的BLV一起培养。供体细胞的增殖受到辐射或丝裂霉素C处理的抑制。在其他实验中，使用来自含有病毒的供体细胞的上清液。与供体细胞共培养时，低分化的单核细胞表现出严重的细胞变化，如分化、空泡化、细胞溶解和膜泡形成;凋亡是一种常见的现象。还观察到芽殖和胞外病毒。分化程度更高的巨噬细胞，尽管它们通过显微镜观察显示较少的感染迹象，但具有完整的BLV蛋白谱，如通过Western印迹所见;清楚地看到对应于p24 CA（Gag）及其前体的条带。此外，gp 51 SU通过合胞体形成测定来鉴定。可以得出结论，M/M细胞可能被BLV感染，感染的后果根据细胞的类型而不同。
The oncogenic retrovirus bovine leukaemia virus (BLV) primarily infects B cells. Most infected animals remain asymptomatic for long periods of time before an increase in circulating B cells or localized tumours can be observed. This long clinical latency period may be explained by cells of the monocyte/macrophage lineage (M/M) becoming infected and acting as a reservoir for the virus, as shown for other retroviruses (human immunodeficiency virus-1, feline immunodeficiency virus). M/M cells in different stages of differentiation (HL-60, THP-1, U-937, J774, BGM, PM2, primary macrophages of sheep and cows) were cultured with BLV produced by permanently infected donor cells (FLKBLV and BLV-bat(2)). Donor cells were inhibited from multiplying by either irradiation or treatment with mitomycin C. In other experiments, supernatant from donor cells containing virus was used. In co-culture with the donor cells, the less differentiated monocytic cells showed severe cellular changes such as differentiation, vacuolization, cell lysis and membrane blebbing; apoptosis was a frequent phenomenon. Budding and extracellular viruses were also observed. The more differentiated macrophage cells, although they showed less signs of infection by microscopy, had a complete BLV protein profile, as seen by Western blotting; bands corresponding to p24CA (Gag) and its precursors were clearly seen. In addition, gp51SU was identified by syncytia formation assays. It is concluded that M/M cells may be infected by BLV, the consequences of the infection differing according to the type of cell.