Hypermethylation of p16INK4a in Korean non-small cell lung cancer patients.
Hypermethylation of p16INK4a in Korean non-small cell lung cancer patients.
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DOI：
10.3346/jkms.2007.22.s.s32
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发表时间：
2007-09
影响因子：
4.5
通讯作者：
Kim JY
中科院分区：
文献类型：
--
作者：
Hong YS;Roh MS;Kim NY;Lee HJ;Kim HK;Lee KE;Kwak JY;Kim JY
采用改良的实时PCR/ SYBR绿色检测方法，对81例韩国原发性肺癌患者的肿瘤组织和癌旁正常组织中p16 INK 4a基因启动子的高甲基化进行了研究。结果显示p16 INK 4a在27.2%的肿瘤组织和11.1%的癌旁正常组织中存在高甲基化。在肿瘤和相应的正常标本中未发现总体异常甲基化之间的显著相关性（r=0.137，p=0.219）。在22例p16 INK 4a高甲基化的肿瘤组织中，仅4例（18.1%）发现有一个高甲基化的正常组织标本。这项研究的结果表明，吸烟可以影响p16 INK 4a启动子区域的甲基化水平，并且这种情况在肿瘤组织中比在正常组织中更常见。其他临床病理特征，包括年龄、性别、肿瘤分期和组织学类型与p16 INK 4a甲基化无关。
Promoter hypermethylation of the p16INK4a gene was investigated in 81 sets of samples of tumor tissue and adjacent normal tissue from Korean patients with primary lung cancer, using the modified real-time polymerase chain reaction (PCR)/ SYBR Green detection method. The results showed hypermethylation of p16INK4a in 27.2% of tumor tissues, and in 11.1% of adjacent normal tissue. No significant association was found between the overall aberrant methylation in tumor and corresponding normal specimens (r=0.137, p=0.219). In 22 cases with p16INK4a hypermethylation in tumor tissues, only 4 (18.1%) cases were found to have a hypermethylated normal tissue specimen. The findings of this study show that smoking can influence the methylation level of the promoter region of p16INK4a, and that this occurs in tumor tissues more frequently than in normal tissues. Other clinicopathological characteristics, including age, sex, tumor stage, and histologic type were not found to be correlated with p16INK4a methylation.