Gastrocnemius mitochondrial respiration: are there any differences between men and women?
Gastrocnemius mitochondrial respiration: are there any differences between men and women?
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腓肠肌线粒体呼吸：男性和女性之间有什么差异吗？
DOI：
10.1016/j.jss.2013.05.054
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Pipinos,IraklisI
中科院分区：
文献类型：
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作者：
Thompson,JonathanR;Swanson,StanleyA;Casale,GeorgeP;Johanning,JasonM;Papoutsi,Evlampia;Koutakis,Panagiotis;Miserlis,Dimitrios;Zhu,Zhen;Pipinos,IraklisI
前言我们等人对人类和小鼠骨骼肌的研究表明，衰老和与年龄相关的退行性疾病与线粒体功能障碍有关，这种功能障碍可能在男性中更常见。然而，还没有专门研究男性或女性对人类骨骼肌线粒体呼吸的影响。本研究的目的是比较成年男性和女性腓肠肌的线粒体呼吸功能。方法取男性(n=19)和女性(n=11)健康的男性和女性的腓肠肌，其下肢肌肉骨骼和动脉系统健康，行走功能正常。所有患者均接受手术治疗腿部静脉曲张。线粒体呼吸是用克拉克电极在含有皂素皮肤的肌束的氧合细胞中测定的。分别测量I、II、III和IV依赖的复杂呼吸，并将其归一化为肌肉重量、总蛋白含量和柠檬酸合成酶(CS，线粒体含量指数)。结果男性和女性患者没有证据表明肌肉骨骼或动脉疾病，年龄、种族、体重指数或其他临床特征没有差异。以肌肉重量、总蛋白含量和CS为标准的I、II、III和IV依赖的复杂呼吸在男性和女性之间没有统计学差异。结论我们的研究首次评估了肌肉骨骼和动脉系统健康、行走功能正常的成年男性和女性的腓肠肌线粒体呼吸。我们的数据表明，腓肠肌线粒体的呼吸在男性和女性之间没有差异。
IntroductionWork on human and mouse skeletal muscle by our group and others has demonstrated that aging and age-related degenerative diseases are associated with mitochondrial dysfunction, which may be more prevalent in males. There have been, however, no studies that specifically examine the influence of male or female sex on human skeletal muscle mitochondrial respiration. The purpose of this study was to compare mitochondrial respiration in the gastrocnemius of adult men and women.MethodsGastrocnemius muscle was obtained from male (n= 19) and female (n= 11) human subjects with healthy lower-extremity musculoskeletal and arterial systems and normal ambulatory function. All patients were undergoing operations for the treatment of varicose veins in their legs. Mitochondrial respiration was determined with a Clark electrode in an oxygraph cell containing saponin-skinned muscle bundles. Complex I-, II-, III-, and IV-dependent respiration was measured individually and normalized to muscle weight, total protein content, and citrate synthase (CS, index of mitochondrial content).ResultsMale and female patients had no evidence of musculoskeletal or arterial disease and did not differ with regard to age, race, body mass index, or other clinical characteristics. Complex I-, II-, III-, and IV-dependent respiration normalized to muscle weight, total protein content, and CS did not statistically differ for males compared with females.ConclusionsOur study evaluates, for the first time, gastrocnemius mitochondrial respiration of adult men and women who have healthy musculoskeletal and arterial systems and normal ambulatory function. Our data demonstrate there are no differences in the respiration of gastrocnemius mitochondria between men and women.