Quantification of optical signals with electrophysiological signals in neural activities of Di-4-ANEPPS stained rat hippocampal slices
Quantification of optical signals with electrophysiological signals in neural activities of Di-4-ANEPPS stained rat hippocampal slices
复制标题
DOI：
10.1016/s0165-0270(00)00270-3
复制
发表时间：
2000-10-15
影响因子：
3
通讯作者：
Ichikawa, M
中科院分区：
文献类型：
--
作者：
Tominaga, T;Tominaga, Y;Ichikawa, M
我们已经量化的光学信号的突触诱导的神经活动在体外脑切片制备的电生理信号。使用电生理学上熟知的用外部施加的荧光电压敏感染料（VSD; Di-4-ANEPPS）染色的大鼠海马切片的CA 1区中的神经活动进行鉴定。连同一个新设计的CCD为基础的数字高速摄像系统和落射荧光光学，我们的改进是在一个协议上染色使用一个新设计的腔室系统。这些改进使我们能够在不影响生理状态的情况下稳定可靠地记录光信号和电生理测量，并在它们之间进行定量比较。光信号的时程和幅度与细胞内和细胞外记录显示出相当的一致性，并且在2 h内稳定。光学信号遵循突触诱导的长时程增强（LTP），由电生理信号监测。在光信号中发现了LTP量的区域差异，并在电生理信号中得到证实。这些结果表明，我们的改进的方法作为一种替代的能力，但更有效的工具来测量脑片的神经元活动，除了电生理方法。(C)2000 Elsevier Science B. V.保留所有权利。
We have quantified the optical signals of synaptically induced neural activities in an in vitro brain slice preparation in terms of electrophysiological signals. The qualification was done using electrophysiologically well known neural activities in the CA1 area of rat hippocampal slices stained with externally applied fluorescent voltage-sensitive dye (VSD; Di-4-ANEPPS). Together with a newly designed CCD-based digital high-speed camera system and epi-fluorescent optics, our improvements were made on a protocol for staining using a newly designed chamber system. These improvements enabled us to make stable and reliable recordings of optical signals and electrophysiological measurements without affecting the physiological status and to make a quantitative comparison between them. The time course and amplitude of the optical signal showed fair agreement with intracellular and extracellular recordings, and was stable over 2 h. The optical signal followed synaptically induced long-term potentiation (LTP) as monitored by the electrophysiological signals. A regional difference in the amount of LTP was found in optical signals and was confirmed in the electrophysiological signals. These results demonstrate the capabilities of our improved method as an alternative but more potent tool to measure the neuronal activities of brain slice in addition to electrophysiological method. (C) 2000 Elsevier Science B.V. All rights reserved.