Stationary Phase Based on β-Cyclodextrin and Poly(N-isopropylacrylamide) for HILIC and RPLC
Stationary Phase Based on β-Cyclodextrin and Poly(N-isopropylacrylamide) for HILIC and RPLC
复制标题
用于 HILIC 和 RPLC 的基于β-环糊精和聚（N-异丙基丙烯酰胺）的固定相
DOI：
10.1007/s10337-015-2989-5
复制
发表时间：
2016-01-01
期刊：
影响因子：
1.7
通讯作者：
Ma, Yulong
中科院分区：
文献类型：
--
作者：
Li, Yuanyuan;Zhu, Nan;Ma, Yulong
以β-环糊精和聚N-异丙基丙烯酰胺为固定相，制备了一种新型固定相。聚合物/100g固定相的质量分数为23%。通过考察流动相中乙腈(ACN)的含量、pH值和柱温对极性和亲水性化合物在富含ACN的流动相中的保留因子的影响，发现新的固定相具有典型的亲水作用液相色谱保留行为。此外，溶质在流动相中的保留曲线呈“U”形，这表明HILIC/反相液相色谱(RPLC)的混合模式保留行为。此外，利用该材料分别在HILIC和RPLC模式下实现了部分亲水性化合物和不同极性化合物的分离。
A new stationary phase based on beta-cyclodextrin and Poly(N-isopropylacrylamide) was prepared. The mass % of the polymer/100 g stationary phase was 23 %. Through investigating the effects of the acetonitrile (ACN) content of the mobile phase, the pH and the column temperature on the retention factor of polar and hydrophilic compounds in the ACN-rich mobile phase, it was found that the new stationary phase had the typical retention behavior of hydrophilic interaction liquid chromatography (HILIC). Moreover, the retention of solutes in the mobile phase exhibited "U-shaped" curves, which was an indication of HILIC/reversed-phase liquid chromatography (RPLC) mixed-mode retention behavior. In addition, the separation of some hydrophilic and different polar compounds was achieved by using the new material in HILIC and RPLC modes, respectively.