Design and build a green tent environment for growing and charactering mycelium growth in lab
Design and build a green tent environment for growing and charactering mycelium growth in lab
复制标题
设计和建造一个绿色帐篷环境，用于实验室中菌丝体的生长和表征
DOI：
10.1039/d3lc00336a
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发表时间：
2023
期刊：
影响因子：
6.1
通讯作者：
Qin, Zhao
中科院分区：
文献类型：
--
作者：
Yang, Libin;Xu, Ruohan;Joardar, Anushka;Amponsah, Michael;Sharifi, Nina;Dong, Bing;Qin, Zhao
近年来，以菌丝体为基础的材料在制造业中越来越受欢迎。本研究旨在建立实验室规模的实验设施，研究菌丝在温度和湿度控制良好的环境下的生长情况，并探讨不同化学和力学性质的基质如何影响菌丝纤维在生长过程中的微观形态。在这里，我们设计并搭建了一个定制的绿色帐篷，它具有良好的隔热保温性能，用于控制温度和湿度，并使用Arduino芯片监测环境数据。我们开发了从孢子到纤维网生长菌丝的程序。结果表明，具有可溶性营养的水凝胶基质比硬木板更适合菌丝体生长，生长速度更快。我们对硬木板和水凝胶基材上的菌丝体纤维进行了许多显微成像，发现直径（~ 3 μm）没有显著差异。本研究为探究菌丝体生长机制及探索其环保、高效的生长方法提供了基础。
Mycelium-based materials have seen a surge in popularity in the manufacturing industry in recent years. This study aims to build a lab-scale experimental facility to investigate mycelium growth under a well-controlled temperature and humidity environment and explore how substrates of very different chemical and mechanical properties can affect the microscopic morphology of the mycelium fibers during growth. Here, we design and build a customized green tent with good thermal and humidity insulation for controlling the temperature and humidity and monitor the environmental data with an Arduino chip. We develop our procedure to grow mycelium from spores to fibrous networks. It is shown that a hydrogel substrate with soluble nutrition is more favorite for mycelium growth than a hardwood board and leads to higher growing speed. We take many microscopic images of the mycelium fibers on the hardwood board and the hydrogel substrate and found no significant difference in diameter (∼3 μm). This research provides a foundation to explore the mechanism of mycelium growth and explore the environmentally friendly and time-efficient method of its growth.