SUPPRESSION OF T-CELL PROLIFERATION BY CD8+ T-CELLS INDUCED IN THE PRESENCE OF PROTOSCOLICES OF ECHINOCOCCUS-MULTILOCULARIS INVITRO
SUPPRESSION OF T-CELL PROLIFERATION BY CD8+ T-CELLS INDUCED IN THE PRESENCE OF PROTOSCOLICES OF ECHINOCOCCUS-MULTILOCULARIS INVITRO
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DOI：
10.1128/iai.61.2.525-533.1993
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发表时间：
1993-02-01
影响因子：
3.1
通讯作者：
ONOE, K
中科院分区：
文献类型：
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作者：
KIZAKI, T;ISHIGE, M;ONOE, K
在体外系统中研究了多房棘球蚴原核细胞（PSC）对小鼠T淋巴细胞功能的免疫调节作用。PSC的加入抑制了伴刀豆球蛋白A（ConA）或抗CD 3单克隆抗体刺激的脾细胞的增殖反应。在PSC的存在下，白细胞介素-2（IL-2）的生产和IL-2受体（IL-2 R）的表达与ConA刺激的淋巴细胞显着减少。然而，外源性IL-2重建ConA刺激的增殖反应和IL-2 R表达。这些结果提示E. multiculocularis可以通过影响IL-2的产生来抑制淋巴细胞的反应。事实上，从用ConA加PSC刺激的培养物中加入富含CD 8+的细胞至新鲜脾细胞显示出对ConA反应的显著抑制。如此处理的脾细胞上的IL-2产生以及IL-2 R表达被抑制。这些结果揭示了E.在一些实施方案中，本发明涉及涉及抑制IL-2产生和降低IL-2 R表达的多房性PSC。PSC诱导的CD 8+细胞似乎在抑制调节宿主对E.多房的
Immunoregulatory influences of protoscolices (PSC) of Echinococcus multilocularis on murine T-lymphocyte functions have been examined in an in vitro system. Proliferative responses of spleen cells stimulated with concanavalin A (ConA) or anti-CD3 monoclonal antibodies were depressed by the addition of PSC. In the presence of PSC, both interleukin-2 (IL-2) production and IL-2 receptor (IL-2R) expression by lymphocytes stimulated with ConA were significantly reduced. However, exogenous IL-2 reconstituted both the ConA-stimulated proliferative responses and IL-2R expression. These findings suggest that PSC of E. multilocularis can suppress lymphoid cell responses via influences on IL-2 production. Indeed, addition of CD8+-enriched cells from cultures stimulated with ConA plus PSC to fresh spleen cells showed marked suppression of the ConA responses. IL-2 production as well as IL-2R expression on the spleen cells so treated were suppressed. These findings reveal a suppressive immunologic function induced by E. multilocularis PSC that involves inhibition of IL-2 production and reduction of IL-2R expression. The PSC-induced CD8+ cells appear to play a key role in the suppressive regulation of host immune responses against E. multilocularis.