Cilia distribution and polarity in the epithelial lining of the mouse middle ear cavity.
Cilia distribution and polarity in the epithelial lining of the mouse middle ear cavity.
复制标题
小鼠中耳腔上皮衬里的纤毛分布和极性。
DOI：
10.1038/srep45870
复制
发表时间：
2017-03-30
影响因子：
4.6
通讯作者：
Chen P
中科院分区：
文献类型：
--
作者：
Luo W;Yi H;Taylor J;Li JD;Chi F;Todd NW;Lin X;Ren D;Chen P
中耳将声音传导到耳蜗以进行听觉。中耳炎（OM）是儿童时期最常见的疾病。此外，慢性渗出性OM（COME）是传导性听力损失的主要原因。临床上，COME与原发性纤毛运动障碍高度相关，表明纤毛功能障碍对COME有重要贡献。然而，对中耳纤毛特性的理解是有限的，这些特性对OM易感性至关重要。在这里，我们证实了在中耳腔腹侧区域的咽鼓管口附近存在纤毛区，主要由多纤毛的管腔层和角蛋白-5阳性基底细胞层组成。我们还发现，运动纤毛的极化协调，并显示一个平面细胞极性。令人惊讶的是，我们还发现了一个多纤毛细胞的区域，该区域排列在中耳腔的后背极，以前认为该区域仅包含非纤毛细胞。我们的研究提供了一个更完整的了解纤毛分布，并首次揭示了哺乳动物中耳上皮细胞中纤毛的协调极性，从而说明了新的结构特征，可能是中耳功能的关键，并与OM易感性。
The middle ear conducts sound to the cochlea for hearing. Otitis media (OM) is the most common illness in childhood. Moreover, chronic OM with effusion (COME) is the leading cause of conductive hearing loss. Clinically, COME is highly associated with Primary Ciliary Dyskinesia, implicating significant contributions of cilia dysfunction to COME. The understanding of middle ear cilia properties that are critical to OM susceptibility, however, is limited. Here, we confirmed the presence of a ciliated region near the Eustachian tube orifice at the ventral region of the middle ear cavity, consisting mostly of a lumen layer of multi-ciliated and a layer of Keratin-5-positive basal cells. We also found that the motile cilia are polarized coordinately and display a planar cell polarity. Surprisingly, we also found a region of multi-ciliated cells that line the posterior dorsal pole of the middle ear cavity which was previously thought to contain only non-ciliated cells. Our study provided a more complete understanding of cilia distribution and revealed for the first time coordinated polarity of cilia in the epithelium of the mammalian middle ear, thus illustrating novel structural features that are likely critical for middle ear functions and related to OM susceptibility.