The Examination of ESAT-6, CFP-10, MPT-64 Antigens of Mycobacterium tuberculosis in Urine of Pediatric Tuberculosis Patient with Immunochromatography to Support the Diagnosis
The Examination of ESAT-6, CFP-10, MPT-64 Antigens of Mycobacterium tuberculosis in Urine of Pediatric Tuberculosis Patient with Immunochromatography to Support the Diagnosis
复制标题
免疫层析法检测小儿结核病患者尿液中结核杆菌ESAT-6、CFP-10、MPT-64抗原辅助诊断
DOI：
10.20473/fmi.v54i3.10014
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发表时间：
2018
影响因子：
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通讯作者：
Agung Dwi Wahyu W
中科院分区：
文献类型：
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作者：
Anita Budiarti;J. Nugraha;Agung Dwi Wahyu W
儿童结核病往往是一种全身性并发症，因为他们的细胞免疫系统不完善。ESAT-6、CFP-10和MPT-64是由Mtb分泌到血液中并由肾脏过滤到尿液中的小分子显性抗原。根据印度尼西亚共和国卫生部的数据，2013年儿童结核病病例的高比例为7.9%-12%。很难通过痰液和血液样本进行诊断。因此，有必要支持使用ICT快速检测尿液样本的诊断。本研究的目的是确定ICT儿童TB患者尿液中Mtb ESAT-6，CFP-10和MPT-64抗原的检查，以支持诊断。方法采用病例对照分析观察法。研究结果显示，32例接受ICT检查的结核病患儿尿液中，22例ESAT-6、CFP-10和MPT-64阳性，10例阴性。而32例健康儿童尿液中，25例阴性，7例阳性。分析结果显示，与儿科医生的结核诊断相比，95%置信度（CI），p = 0.0002（p <0.05），78%特异性和68.8%敏感性。因此，Mtb ESAT-6，CFP-10和MPT-64抗原可以在ICT儿童TB患者的尿液中进行检查，以支持诊断。
Tuberculosis in children is often a systemic complication because of their imperfect cellular immune system. ESAT-6, CFP-10, and MPT-64 are small molecular dominant antigens secreted by Mtb into the blood and filtered by the kidneys into urine. The high proportion of children with TB cases according to the Ministry of Health of the Republic of Indonesia in 2013 was 7.9%-12%. Diagnis is difficult to establish with sputum and blood samples. Therefore, it is necessary to support the diagnosis using urine samples with ICT rapid test. The purpose of this study was to determine the examination of Mtb ESAT-6, CFP-10, and MPT-64 antigens in the urine of pediatric TB patients with ICT to support the diagnosis. The method used was analytic observation with case control design. The results of the study showed that 32 of the urine of children with TB who were examined with ICT, 22 showed ESAT-6, CFP-10, and MPT-64 positive and 10 negative. Whereas, from 32 urine of healthy children, 25 were negative and 7 were positive. Analysis results showed 95% confidence (CI), p = 0.0002 (p <0.05), 78% specificity and 68.8% sensitivity compared to TB diagnosis from pediatricians. As a conclusion, Mtb ESAT-6, CFP-10, and MPT-64 antigens can be examined in the urine of pediatric TB patients with ICT to support the diagnosis.