ENHANCED TRANSCRIPTION OF MITOCHONDRIAL GENES AFTER GROWTH-STIMULATION AND GLUCOCORTICOID TREATMENT OF REUBER HEPATOMA H-35
ENHANCED TRANSCRIPTION OF MITOCHONDRIAL GENES AFTER GROWTH-STIMULATION AND GLUCOCORTICOID TREATMENT OF REUBER HEPATOMA H-35
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DOI：
10.1016/0014-5793(88)81354-1
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发表时间：
1988-06-06
期刊：
影响因子：
3.5
通讯作者：
KITAGAWA, Y
中科院分区：
文献类型：
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作者：
KADOWAKI, T;KITAGAWA, Y
当用血清刺激去血清培养的人肝癌H-35细胞时，细胞同步增殖，在16 h内出现[3 H]胸苷掺入高峰，24 h内细胞数倍增。用大鼠线粒体DNA克隆片段作为探针，通过细胞总RNA的北方杂交，观察到线粒体基因mRNA在S期前增加5-10倍。从生长停滞或生长刺激的细胞中分离的线粒体的细胞器转录表明，线粒体mRNA的增加主要是由于转录增强。用地塞米松处理细胞观察到较少的转录增强，这导致大量蛋白质易位到线粒体中。后者的作用被胰岛素抵消。
When a serum‐deprived culture of Reuber hepatoma H‐35 cells was stimulated by serum, cells proliferated synchronously showing a peak of [3H]thymidine incorporation within 16 h and doubling of cell number within 24 h. A 5–10‐fold increase of mRNAs of mitochondrial genes was observed prior to S‐phase by Northern hybridization of total cellular RNA with cloned fragments of rat mitochondrial DNA as probes. In organelle transcription by mitochondria isolated from growth‐arrested or growth‐stimulated cells suggested that the increase of mitochondrial mRNAs was mainly due to enhanced transcription. Less enhanced transcription was observed by treating the cells with dexamethasone, which causes translocation of large amount of protein into mitochondria. The latter effect was counteracted by insulin.