Quantitative analysis of florigen for the variability of floral induction in cabbage/radish inter-generic grafting
Quantitative analysis of florigen for the variability of floral induction in cabbage/radish inter-generic grafting
复制标题
成花素定量分析甘蓝/萝卜属间嫁接花诱导变异性
DOI：
10.1093/pcp/pcac098
复制
发表时间：
2022
影响因子：
4.9
通讯作者：
Nakazaki T
中科院分区：
文献类型：
--
作者：
Motoki K;Kinoshita Y;Nakano R;Hosokawa M;Nakazaki T
嫁接诱导开花是理解成花素诱导系统成花的关键现象。它还可以作为一种育种技术，使具有长世代时间的作物能够快速生产种子。然而，嫁接植株的成花诱导程度往往是不同的。此外，在一些作物品种中很难做到这一点。在此，我们探索了促进甘蓝嫁接诱导开花变异的因素。通过对成花素积累的定量分析，确定了花原素的积累规律。不同类型砧木嫁接甘蓝的开花反应存在显著差异。据报道，甘蓝砧木不能诱导嫁接的甘蓝植株开花，但萝卜(Raphanus sativusL.)砧木的表现不稳定，这取决于所使用的材料。免疫印迹分析表明，成花素的主要成分开花位点T(FT)蛋白的诱导程度与嫁接接穗中FT蛋白的积累量呈定量相关。为了确定嫁接接穗成花诱导过程中引起变异的砧木因素，我们研究了春化、日长和修叶处理改变嫁接接穗FT蛋白转录水平和砧木叶面积时嫁接接穗中FT蛋白的积累和开花反应。我们认为，增加砧木中FT蛋白的总量对于嫁接甘蓝稳定的成花诱导是重要的，这可以通过增加FT转录和砧木的叶面积来实现。
Grafting-induced flowering is a key phenomenon to understand systemic floral induction caused by florigen. It can also be used as a breeding technique enabling rapid seed production of crops with long generation times. However, the degree of floral induction in grafted plants is often variable. Moreover, it is difficult in some crop species. Here, we explored the factors promoting variability in the grafting-induced flowering of cabbage (Brassica oleraceaL. var.capitata), an important vegetable crop with a long generation time, via the quantitative analysis of florigen accumulation. Significant variability in the flowering response of grafted cabbage was observed when rootstocks of different genotypes were used. As reported previously,B. oleracearootstocks did not induce the flowering of grafted cabbage plants, but radish (Raphanus sativusL.) rootstocks unstably did, depending on the accessions used. Immunoblotting analysis of the FLOWERING LOCUS T (FT) protein, a main component of florigen, revealed that floral induction was quantitatively correlated with the level of accumulated FT protein in the grafted scion. To identify rootstock factors that cause variability in the floral induction of the grafted scion, we investigated FT protein accumulation and flowering response in grafted scions when the transcription levels ofFTand the leaf area of rootstocks were altered by vernalization, daylength and leaf trimming treatments. We concluded that increasing the total amount of FT protein produced in the rootstock is important for the stable floral induction of the grafted cabbage, and this can be accomplished by increasingFTtranscription and the leaf area of the rootstock.