Well-controlled proinflammatory cytokine responses of Peyer's patch cells to probiotic Lactobacillus casei
Well-controlled proinflammatory cytokine responses of Peyer's patch cells to probiotic Lactobacillus casei
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DOI：
10.1111/j.1365-2567.2009.03204.x
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发表时间：
2010-07-01
期刊：
影响因子：
6.4
通讯作者：
Nomoto, Koji
中科院分区：
文献类型：
--
作者：
Chiba, Yukihide;Shida, Kan;Nomoto, Koji
为了阐明益生菌的免疫调节特征，检测了小鼠脾和派伊尔集合淋巴结（PP）细胞响应益生菌和病原菌的细胞因子产生。在脾细胞中，益生菌干酪乳杆菌诱导CD 11b+细胞产生的白细胞介素（IL）-12比致病性革兰氏阳性和革兰氏阴性细菌更强，并有效地促进T辅助细胞（Th）1型细胞的发育，随后分泌高水平的干扰素（IFN）-γ。虽然PP细胞对L.酪蛋白的表达水平低于脾细胞，Th 1细胞由于这种低水平的IL-12诱导而发育。然而，L.用特异性抗原刺激的酪蛋白诱导的Th 1细胞在PP细胞中下调。通过抗原刺激在PP细胞中有效诱导产生IL-17的Th 17细胞的发育。干酪乳杆菌轻微但显著地抑制抗原诱导的IL-17分泌，而不降低Th 17细胞的比例。没有测试的细菌诱导产生IL-10、产生转化生长因子β或表达Foxp 3的调节性T细胞的发育，因此表明某些益生菌可能通过PP细胞中尚未鉴定的机制调节促炎反应。这些数据显示益生菌L.酪蛋白具有相当大的诱导IL-12产生和促进Th 1细胞发育的潜力，但PP细胞中促炎细胞因子如IL-12和IL-17的分泌可能受到很好的控制。
P>In order to clarify the probiotic features of immunomodulation, cytokine production by murine spleen and Peyer's patch (PP) cells was examined in response to probiotic and pathogenic bacteria. In spleen cells, probiotic Lactobacillus casei induced interleukin (IL)-12 production by CD11b+ cells more strongly than pathogenic Gram-positive and Gram-negative bacteria and effectively promoted the development of T helper (Th) type 1 cells followed by high levels of secretion of interferon (IFN)-gamma. Although the levels of IL-12 secreted by PP cells in response to L. casei were lower in comparison with spleen cells, Th1 cells developed as a result of this low-level induction of IL-12. However, IFN-gamma secretion by the L. casei-induced Th1 cells stimulated with a specific antigen was down-regulated in PP cells. Development of IL-17-producing Th17 cells was efficiently induced in PP cells by antigen stimulation. Lactobacillus casei slightly, but significantly, inhibited the antigen-induced secretion of IL-17 without a decrease in the proportion of Th17 cells. No bacteria tested induced the development of IL-10-producing, transforming growth factor-beta-producing or Foxp3-expressing regulatory T cells, thus suggesting that certain probiotics might regulate proinflammatory responses through as yet unidentified mechanisms in PP cells. These data show probiotic L. casei to have considerable potential to induce IL-12 production and promote Th1 cell development, but the secretion of proinflammatory cytokines such as IL-12 and IL-17 may be well controlled in PP cells.