Acute alterations of glutamate, glutamine, GABA, and other amino acids after spinal cord contusion in rats
Acute alterations of glutamate, glutamine, GABA, and other amino acids after spinal cord contusion in rats
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DOI：
10.1007/s11064-006-9225-5
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Rios, Camilo
中科院分区：
文献类型：
--
作者：
Diaz-Ruiz, Araceli;Salgado-Ceballos, Hermelinda;Rios, Camilo
脊髓损伤导致能量代谢的改变。因此，谷氨酸、谷氨酰胺、天冬氨酸等重要氨基酸在损伤后发生改变，导致重要的神经化学通路失调。在本研究中，我们描述了大鼠脊髓挫伤后参与神经递质和非神经递质作用的氨基酸组织浓度的急性期变化。分别于损伤后2、4、8、12小时处死，用HPLC法分析组织氨基酸。结果表明，脊髓损伤后，天冬氨酸和谷氨酸含量均下降，谷氨酰胺浓度升高，但谷氨酸和谷氨酰胺的摩尔浓度之和在各时间点保持不变。与对照组相比，GABA浓度增加，而甘氨酸保持不变。最后，瓜氨酸水平因脊髓损伤而升高，而牛磺酸仅在损伤后4小时升高。结果表明，脊髓损伤后氨基酸浓度发生了复杂的急性期变化，反映了损伤后不同的损伤过程。
Spinal cord injury (SCI) leads to an alteration of energetic metabolism. As a consequence, glutamate, glutamine, aspartate and other important amino acids are altered after damage, leading to important disregulation of the neurochemical pathways. In the present study, we characterized the acute-phase changes in tissue concentration of amino acids involved in neurotransmitter and non-neurotransmitter actions after SCI by contusion in rats. Animals were submitted to either laminectomy or SCI by contusion and sacrificed at 2, 4, 8, and 12 h after lesion, for the analysis of tissue amino acids by HPLC. Results showed that both aspartate and glutamate contents diminished after SCI, while glutamine concentrations raised, however, the sum of molar concentrations of glutamate plus glutamine remained unchanged at all time points. GABA concentrations increased versus control group, while glycine remained unchanged. Finally, citrulline levels increased by effect of SCI, while taurine-increased only 4 h after lesion. Results indicate complex acute-phase changes in amino acids concentrations after SCI, reflecting the different damaging processes unchained after lesion.