In Vitro Differentiation of Mouse Granulocytes.
In Vitro Differentiation of Mouse Granulocytes.
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DOI：
10.1007/978-1-4939-3581-9_8
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发表时间：
2016
影响因子：
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通讯作者：
Ramona Reinhart;S. Wicki;T. Kaufmann
中科院分区：
文献类型：
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作者：
Ramona Reinhart;S. Wicki;T. Kaufmann
粒细胞是免疫系统的核心角色，一旦被激活，在效应功能和细胞凋亡清除之间的严格控制平衡保证了最大的宿主利益，对健康组织的附带损害最小。粒细胞是终末分化的细胞，不能在培养中维持很长时间。在小鼠实验中，分离原发性粒细胞效率低下且具有挑战性，特别是对于低丰度的嗜酸性粒细胞和嗜碱性粒细胞亚型。在这里，我们描述了一种体外方案，大规模扩增小鼠来源的髓系祖细胞，并“按需”将它们大量分化为成熟的中性粒细胞或嗜碱性粒细胞。
Granulocytes are central players of the immune system and, once activated, a tightly controlled balance between effector functions and cell removal by apoptosis guarantees maximal host benefit with least possible collateral damage to healthy tissue.Granulocytes are terminally differentiated cells that cannot be maintained in culture for prolonged times. Isolating primary granulocytes is inefficient and challenging when working with mice, and especially so for the lowly abundant eosinophil and basophil subtypes. Here we describe an in vitro protocol to massively expand mouse derived myeloid progenitors and to differentiate them “on demand” and in large numbers into mature neutrophils or basophils.