Immunochemical characterization of the basement membrane glycoprotein laminin.
Immunochemical characterization of the basement membrane glycoprotein laminin.
复制标题
基底膜糖蛋白层粘连蛋白的免疫化学特征。
DOI：
--
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
R. Timpl
中科院分区：
文献类型：
--
作者：
H. Rohde;G. Wick;R. Timpl
层粘连蛋白最近被鉴定为由小鼠肿瘤产生的基底膜基质的主要非胶原蛋白。用中性缓冲液从肿瘤基质中提取，并在非变性条件下纯化。兔和豚鼠抗血清提出的层粘连蛋白或一个大的胃蛋白酶片段P1层粘连蛋白表现出很强的结合层粘连蛋白和肽P1，但只有一个弱的反应，减少和烷基化层粘连蛋白。层粘连蛋白的主要抗原决定簇位于二硫键结中，占分子的三分之一，可抵抗胃蛋白酶或溴化氰的降解。还可以鉴定天然蛋白和还原蛋白共有的次要抗原决定簇。这些数据被解释为显示层粘连蛋白由构象刚性以及更柔性的结构域组成。小鼠肾匀浆的吸收研究表明，真正的基底膜含有免疫学上与层粘连蛋白相同的蛋白质。来自其他物种的组织含有与小鼠层粘连蛋白表现出部分交叉反应的相关蛋白。与免疫荧光数据一起，研究结果表明，层粘连蛋白，像IV型胶原蛋白，发生在大多数基底膜的身体。
Laminin was recently characterized as being a major non-collageneous protein of the basement membrane matrix produced by a mouse tumor. It was extracted from the tumor matrix with neutral buffer and purified under non-denaturing conditions. Rabbit and guinea pig antisera raised against laminin or a large pepsin fragment P1 of laminin showed strong binding to both laminin and peptide P1 but only a weak reaction with reduced and alkylated laminin. The major antigenic determinants of laminin were located in a disulfide knot, comprising one third of the molecule, which resisted degradation by pepsin or cyanogen bromide. Minor antigenic determinants shared by the native and reduced protein could also be identified. The data were interpreted as showing that laminin consists of conformationally rigid as well as more flexible domains. Absorption studies with mouse kidney homogenate indicated that authenic basement membranes contain a protein immunologically identical to laminin. Tissues from other species contain a related protein which exhibits partial cross-reaction with mouse laminin. Together with immunofluorescence data the findings demonstrate that laminin, like type IV collagen, occurs in most basement membranes of the body.