A minimally invasive lens-free computational microendoscope
A minimally invasive lens-free computational microendoscope
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DOI：
10.1126/sciadv.aaw5595
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发表时间：
2019-12
期刊：
影响因子：
13.6
通讯作者：
Jaewook Shin;D. Tran;J. R. Stroud;S. Chin;T. Tran;M. Foster
中科院分区：
文献类型：
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作者：
Jaewook Shin;D. Tran;J. R. Stroud;S. Chin;T. Tran;M. Foster
研制了一种远端无透镜显微内窥镜，以实现大视场和数字重聚焦的微创成像。超小型显微内窥镜对于许多生物医学应用是至关重要的。这种微创成像仪可以导航到难以触及的区域，并观察自由活动的动物的深层大脑活动。传统的解决方案使用远端微透镜。然而，随着镜片变得更小，侵入性更小，它们会产生更大的像差和视野限制。此外，大多数能够可变对焦的成像器需要对镜头进行机械驱动，这增加了远端的复杂性和重量。在这里，我们展示了一种通过计算图像恢复实现显微内窥镜检查的远端无透镜方法。我们的方法是完全无激励的，并且在多芯光纤的远端使用单个伪随机空间掩模。在实验上，这种无透镜的方法将空间带宽乘积，即视场除以分辨率，比基于最佳透镜的系统增加了三倍。此外，显微内窥镜演示了彩色分辨率成像和从单个相机框架重新聚焦到11个不同的深度平面，而没有任何致动部件。
A distal lensless microendoscope is developed to enable minimally invasive imaging with wide field of view and digital refocusing. Ultra-miniaturized microendoscopes are vital for numerous biomedical applications. Such minimally invasive imagers allow for navigation into hard-to-reach regions and observation of deep brain activity in freely moving animals. Conventional solutions use distal microlenses. However, as lenses become smaller and less invasive, they develop greater aberrations and restricted fields of view. In addition, most of the imagers capable of variable focusing require mechanical actuation of the lens, increasing the distal complexity and weight. Here, we demonstrate a distal lens-free approach to microendoscopy enabled by computational image recovery. Our approach is entirely actuation free and uses a single pseudorandom spatial mask at the distal end of a multicore fiber. Experimentally, this lensless approach increases the space-bandwidth product, i.e., field of view divided by resolution, by threefold over a best-case lens-based system. In addition, the microendoscope demonstrates color resolved imaging and refocusing to 11 distinct depth planes from a single camera frame without any actuated parts.