Fluorescence energy transfer between Acridine Orange and Safranine T and its application in the determination of DNA
Fluorescence energy transfer between Acridine Orange and Safranine T and its application in the determination of DNA
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DOI：
10.1016/s0039-9140(98)00386-5
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发表时间：
1999-06-14
期刊：
影响因子：
6.1
通讯作者：
Peng, L
中科院分区：
文献类型：
--
作者：
Cao, Y;He, XW;Peng, L
研究了DNA嵌入剂吖啶橙子和藏红T之间的荧光能量转移。吖啶橙子和藏红T之间的FET效率在插入状态下比在自由状态下更高，并且临界距离R-0更长。提出了一种测定小牛胸腺DNA（ctDNA）的新方法。ctDNA的线性范围为（0 ~ 1.1）× 10（-5）mol l（-1），检测限为2.6 × 10（-7）mol l（-1）。(C)1999 Elsevier Science B. V.保留所有权利。
The fluorescence energy transfer (FET) between Acridine Orange and Safranine T, two intercalators of DNA, was studied in this paper. The FET efficiency between Acridine Orange and Safranine T is higher and the critical distance, R-0, is longer in the intercalated state than in the free one. A new method for the determination of calf thymus DNA (ctDNA) was presented. The linear range of the calibration curve is (0 similar to 1.1) x 10(-5) mol l(-1) in bases for ctDNA, and the limit of detection is 2.6 x 10(-7) mol l(-1). (C) 1999 Elsevier Science B.V. All rights reserved.