Neuronal plasticity in aging: A quantitative immunohistochemical study of GAP-43 distribution in discrete regions of the rat brain
Neuronal plasticity in aging: A quantitative immunohistochemical study of GAP-43 distribution in discrete regions of the rat brain
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DOI：
10.1016/0006-8993(95)01504-3
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发表时间：
1996-04-01
期刊：
影响因子：
2.9
通讯作者：
BertoniFreddari, C
中科院分区：
文献类型：
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作者：
Casoli, T;Spagna, C;BertoniFreddari, C
神经可塑性的神经生长相关蛋白GAP-43被认为是神经细胞结构适应能力的标志物。我们进行了定量免疫组织化学研究的GAP-43的分布在分子层的小脑皮质，在海马齿状回的内分子层，在放射层的CA 1区，在扣带回皮质的第1层和神经纤维层的主要嗅球的3个月，18个月和31个月的Wistar大鼠。GAP-43免疫反应性的减少，观察到在老年大鼠与成年动物相比，在所有5个领域的分析，虽然这些变化仅在齿状回，扣带皮层和嗅球的统计学显着。在这些区域中，GAP-43免疫标记在老年组与成年组中分别减少了54%、42%和38%。这些数据与年龄相关的神经元密度的减少支配的领域进行了比较，我们支持的假设，GAP-43的下降观察到在老年动物的文件一致减少轴突可塑性的内部分子层的齿状回和扣带皮层的第1层。这些结果表明GAP-43作为突触可塑性的年龄依赖性恶化的标志物的重要作用，特别是在涉及记忆和情感行为的大脑区域。
Age-related changes in neuroplasticity have been investigated considering the neuronal growth-associated protein GAP-43 as a marker of nerve cell structural adaptive capabilities. We carried out a quantitative immunohistochemical study on the distribution of GAP-43 in the molecular layer of the cerebellar cortex, in the inner molecular layer of the hippocampal dentate gyrus, in the stratum radiatum of the CA1 region, in layer 1 of the cingulate cortex and in the nerve fiber layer of the main olfactory bulb of 3-, 18- and 31-month-old Wistar rats. A decrease of GAP-43 immunoreactivity was observed in the old rats in comparison with the adult animals in all the 5 areas analyzed, although these variations were only statistically significant in the dentate gyrus, cingulate cortex and olfactory bulb. In these latter zones, GAP-43 immunolabeling is reduced by 54, 42 and 38%, respectively, in the old versus the adult group. Comparing these data with the age-dependent decrease of neuron density innervating the areas investigated, we support the hypothesis that the decline of GAP-43 observed in old animals documents a consistent reduction of axon plasticity in the inner molecular layer of the dentate gyrus and in layer 1 of the cingulate cortex. These results suggest an important role of GAP-43 as a marker of age-dependent deterioration of synaptic plasticity, especially in those areas of the brain involved in memory and emotional behavior.