Association of Nuclear PIM1 Expression with Lymph Node Metastasis and Poor Prognosis in Patients with Lung Adenocarcinoma and Squamous Cell Carcinoma.
Association of Nuclear PIM1 Expression with Lymph Node Metastasis and Poor Prognosis in Patients with Lung Adenocarcinoma and Squamous Cell Carcinoma.
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肺腺癌和鳞状细胞癌患者核 PIM1 表达与淋巴结转移和不良预后的关系
DOI：
10.7150/jca.13422
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发表时间：
2016
影响因子：
3.9
通讯作者：
Li K
中科院分区：
文献类型：
--
作者：
Jiang R;Wang X;Jin Z;Li K
越来越多的证据表明PIM1、p-STAT3和c-MYC的异常表达参与多种实体瘤的发病机制，但其在非小细胞肺癌（NSCLC）中的预后价值仍不清楚。在这里，我们试图评估这些标志物在肺腺癌（AD）和鳞状细胞癌（SCC）患者中的表达和预后作用。采用实时RT-PCR和Western blotting分别分析NSCLC细胞系中PIM1的mRNA和蛋白表达。对 194 名肺 AD 和 SCC 患者的档案肿瘤样本中 PIM1、p-STAT3 和 c-MYC 的表达进行了免疫组织化学检测。在 43.3% 的 AD 和 SCC 中检测到高核 PIM1 表达，并与淋巴结 (LN) 转移 (P = 0.028) 和组织学 (P = 0.003) 显着相关。高核PIM1表达（P=0.034）、局部晚期（P<0.001）、AD（P=0.007）和较差的病理分化（P=0.002）与较差的无病生存（DFS）相关。核PIM1高表达（P = 0.009）、临床分期晚期（P < 0.001）和病理分化差（P = 0.004）是总生存（OS）的独立不利预后因素。 p-STAT3 高表达与 OS 无关，但与 LN 转移显着相关，而 c-MYC 与任何临床病理参数或生存率均不显着相关。因此，在 AD 和 SCC 患者中，核 PIM1 表达水平是 DFS 和 OS 的独立因素，并且可以作为结果的预测生物标志物。
Increasing evidence indicates that aberrant expression of PIM1, p-STAT3 and c-MYC is involved in the pathogenesis of various solid tumors, but its prognostic value is still unclear in non-small cell lung cancer (NSCLC). Here, we sought to evaluate the expression and prognostic role of these markers in patients with lung adenocarcinoma (AD) and squamous cell carcinoma (SCC). Real time RT-PCR and Western blotting was used to analyze the mRNA and protein expression of PIM1 in NSCLC cell lines, respectively. The expression of PIM1, p-STAT3, and c-MYC was immunohistochemically tested in archival tumor samples from 194 lung AD and SCC patients. High nuclear PIM1 expression was detected in 43.3% of ADs and SCCs, and was significantly correlated with lymph node (LN) metastasis (P = 0.028) and histology (P = 0.003). High nuclear PIM1 expression (P = 0.034), locally advanced stage (P < 0.001), AD (P = 0.007) and poor pathologic differentiation (P = 0.002) were correlated with worse disease-free survival (DFS). High nuclear PIM1 expression (P = 0.009), advanced clinical stage (P < 0.001) and poor pathologic differentiation (P = 0.004) were independent unfavorable prognostic factors for overall survival (OS). High p-STAT3 expression was not associated with OS but significantly correlated with LN metastasis, while c-MYC was not significantly correlated with any clinicopathological parameter or survival. Therefore, in AD and SCC patients, nuclear PIM1 expression level is an independent factor for DFS and OS and it might serve as a predictive biomarker for outcome.