A dual-modal magnetic nanoparticle probe for preoperative and intraoperative mapping of sentinel lymph nodes by magnetic resonance and near infrared fluorescence imaging.
A dual-modal magnetic nanoparticle probe for preoperative and intraoperative mapping of sentinel lymph nodes by magnetic resonance and near infrared fluorescence imaging.
复制标题
DOI：
10.1177/0885328212437883
复制
发表时间：
2013-07
影响因子：
2.9
通讯作者：
Mao H
中科院分区：
文献类型：
--
作者：
Zhou Z;Chen H;Lipowska M;Wang L;Yu Q;Yang X;Tiwari D;Yang L;Mao H
可靠地检测前哨淋巴结以进行前哨淋巴结活检和淋巴结切除术的能力对于转移性癌症患者的临床管理非常重要。然而，使用可见染料的传统前哨淋巴结标测受到光的穿透深度和染料的快速清除的限制。另一方面，使用放射性核苷酸技术进行前哨淋巴结定位本质上空间分辨率较低，并且在前哨淋巴结定位过程中不提供解剖细节。这项工作报告了一种双模态成像探针的开发，该探针具有磁共振和近红外成像功能，使用与发射波长为 830 nm 的近红外分子结合的磁性氧化铁纳米颗粒（10 nm 核心尺寸）进行前哨淋巴结绘图。磁性氧化铁纳米粒子在前哨淋巴结中的积累会产生强烈的 T2 加权磁共振成像对比度，可用于前哨淋巴结的术前定位，而共轭近红外分子则提供具有高信背景比的淋巴结光学成像跟踪。基于新型磁性纳米粒子的双成像探针表现出显着更长的淋巴结保留时间。缀合近红外染料的纳米颗粒发出的近红外信号在前哨淋巴结中可持续长达 60 分钟，而游离近红外染料在小鼠模型中的近红外信号只能持续 25 分钟。此外，鉴于其清除速度慢且灵敏度足够，除了前哨淋巴结外，还可以使用该探头观察腋窝淋巴结。因此，这种具有组织穿透性和前哨淋巴结灵敏检测功能的新型双模态成像探头可应用于术前磁共振成像淋巴结检查，并允许使用近红外光学设备进行术中前哨淋巴结测绘。
The ability to reliably detect sentinel lymph nodes for sentinel lymph node biopsy and lymphadenectomy is important in clinical management of patients with metastatic cancers. However, the traditional sentinel lymph node mapping with visible dyes is limited by the penetration depth of light and fast clearance of the dyes. On the other hand, sentinel lymph node mapping with radionucleotide technique has intrinsically low spatial resolution and does not provide anatomic details in the sentinel lymph node mapping procedure. This work reports the development of a dual modality imaging probe with magnetic resonance and near infrared imaging capabilities for sentinel lymph node mapping using magnetic iron oxide nanoparticles (10 nm core size) conjugated with a near infrared molecule with emission at 830 nm. Accumulation of magnetic iron oxide nanoparticles in sentinel lymph nodes leads to strong T2 weighted magnetic resonance imaging contrast that can be potentially used for preoperative localization of sentinel lymph nodes, while conjugated near infrared molecules provide optical imaging tracking of lymph nodes with a high signal to background ratio. The new magnetic nanoparticle based dual imaging probe exhibits a significant longer lymph node retention time. Near infrared signals from nanoparticle conjugated near infrared dyes last up to 60 min in sentinel lymph node compared to that of 25 min for the free near infrared dyes in a mouse model. Furthermore, axillary lymph nodes, in addition to sentinel lymph nodes, can be also visualized with this probe, given its slow clearance and sufficient sensitivity. Therefore, this new dual modality imaging probe with the tissue penetration and sensitive detection of sentinel lymph nodes can be applied for preoperative survey of lymph nodes with magnetic resonance imaging and allows intraoperative sentinel lymph node mapping using near infrared optical devices.