Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions
Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions
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DOI：
10.1016/j.ijmm.2017.02.003
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发表时间：
2017-04-01
影响因子：
4.1
通讯作者：
Smajs, David
中科院分区：
文献类型：
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作者：
Micenkova, Lenka;Benova, Alzbeta;Smajs, David
大肠杆菌是血流感染和社区获得性败血症的最常见原因。本研究的主要目的是测定大肠杆菌的毒力特性。大肠杆菌分离物来自患有泌尿生殖道、消化系统、肿瘤性血液病或其它病症的原发性疾病的患者的血培养物。结果表明，314 E.从血培养分离的大肠杆菌与先前发表的1283例粪便大肠杆菌的分析数据进行了比较。大肠杆菌分离株。314 E.大肠杆菌分离株包括系统发育群（A、B1、B2、D、C、E和F）的确定、21个毒力因子的鉴定以及30个细菌素编码决定簇。采用脉冲场凝胶电泳分析血培养分离株的克隆特征。急诊血培养分离的大肠杆菌菌株以B2群（59.9%）和D群（21.0%）为主，其次为A群（10.2%）和B1群（5.7%）。常见的毒力因子包括粘附素（fimA 92.0%，pap 47.1%和sfa 26.8%）和铁摄取编码基因（fyuA 87.9%，fepC 79.6%，aer 70.7%，iucC 68.2%和ireA 13.7%），其次是大肠杆菌素（pits岛31.5%）和细胞毒性坏死因子（cnfl 11.1%）。与粪便分离株相比，在血培养衍生的分离株中发现了更高频率的微菌素生产者（和微菌素M决定簇）和更低频率的大肠杆菌素Ib和微菌素B17。E.血培养大肠杆菌的毒力基因比粪培养大肠杆菌的毒力基因多。大肠杆菌分离株。此外，血培养E.与来自血液和胃肠道疾病患者的粪便分离株和血培养分离株相比，来自与泌尿生殖道相关的初步诊断患者的大肠杆菌分离株明显不同，并且在这些分离株中检测到更多的毒力基因。(C)2017 Elsevier GmbH. All rights reserved.
Escherichia coli is the most common cause of bloodstream infections and community-acquired sepsis. The main aim of this study was to determine virulence characteristics of E. coli isolates from hemocultures of patients with a primary disease of urogenital tract, digestive system, a neoplastic blood disease, or other conditions. Results from a set of 314 E. coli isolates from hemocultures were compared to data from a previously published analysis of 1283 fecal commensal E. coli isolates. Genetic profiling of the 314 E. coli isolates involved determination of phylogenetic group (A, B1, B2, D, C, E, and F), identification of 21 virulence factors, as well as 30 bacteriocin-encoding determinants. Pulsed-field gel electrophoresis was used to analyze clonal character of the hemoculture-derived isolates. The E. coli isolates from hemocultures belonged mainly to phylogenetic groups B2 (59.9%) and D (21.0%), and less frequently to phylogroups A (10.2%) and B1 (5.7%). Commonly detected virulence factors included adhesins (fimA 92.0%, pap 47.1%, and sfa 26.8%), and iron-uptake encoding genes (fyuA 87.9%, fepC 79.6%, aer 70.7%, iucC 68.2%, and ireA 13.7%), followed by colibactin (pits island 31.5%), and cytotoxic necrotizing factor (cnfl 11.1%). A higher frequency of microcin producers (and microcin M determinant) and a lower frequency of colicin Ib and microcin B17 was found in hemoculture-derived isolates compared to commensal fecal isolates. E. coli isolates from hemocultures harbored more virulence genes compared to fecal E. coli isolates. In addition, hemoculture E. coli isolates from patients with primary diagnosis related to urogenital tract were clearly different and more virulence genes were detected in these isolates compared to both fecal isolates and hemoculture-derived isolates from patients with blood and gastrointestinal diseases. (C) 2017 Elsevier GmbH. All rights reserved.