STATUS OF MAREKS-DISEASE VIRUS IN ESTABLISHED LYMPHOMA CELL-LINES - HERPESVIRUS INTEGRATION IS COMMON
STATUS OF MAREKS-DISEASE VIRUS IN ESTABLISHED LYMPHOMA CELL-LINES - HERPESVIRUS INTEGRATION IS COMMON
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DOI：
10.1128/jvi.67.1.82-92.1993
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发表时间：
1993-01-01
影响因子：
5.4
通讯作者：
HAMMERSCHMIDT, W
中科院分区：
文献类型：
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作者：
DELECLUSE, HJ;HAMMERSCHMIDT, W
对鸡和火鸡马立克氏病淋巴瘤细胞系进行了马立克氏病病毒（MDV）DNA的检测。在转化的T细胞系和B细胞系中，可以通过常规的Southern印迹杂交、Gardella凝胶电泳以及中期和间期染色体的原位杂交检测到病毒DNA。在所有细胞系中均观察到病毒DNA整合到宿主细胞染色体中。在中期染色体上可检测到2 ~ 12个病毒DNA整合位点。的整合位点的特征为个别细胞系，并优先位于大，中型染色体或微型染色体的端粒。在六个细胞系中的四个中，潜伏感染细胞的少数群体支持MDV的裂解周期，产生线性病毒体DNA。在其中一个细胞系中，可以检测到第三种MDV DNA，其性质让人想起共价闭合的环状DNA。MDV有规律地整合到潜伏感染细胞的基因组中的发现对于理解天然宿主中疱疹病毒诱导的肿瘤的分子生物学至关重要。
Six cell lines derived from Marek's disease lymphomas of chickens and turkeys were investigated for the status of Marek's disease virus (MDV) DNA. In the transformed T- and B-cell lines, viral DNA could be detected by conventional Southern blot hybridization, by Gardella gel electrophoresis, and by in situ hybridization of metaphase and interphase chromosomes. Integration of viral DNA into the host cell chromosome was observed in all cell lines. Two to 12 integration sites of viral DNA could be detected in metaphase chromosome spreads. The integration sites were characteristic for the individual cell lines and were preferentially located at the telomers of large- and mid-sized chromosomes or on minichromosomes. In four of six cell lines, a minor population of latently infected cells supported the lytic cycle of MDV, giving rise to linear virion DNAs. In one of these cell lines, a third species of MDV DNA could be detected with properties reminiscent of covalently closed circular DNA. The finding that MDV integrates regularly into the genomes of latently infected cells is crucial to understanding the molecular biology of herpesvirus-induced tumors in the natural host.