TEX101, a glycoprotein essential for sperm fertility, is required for stable expression of Ly6k on testicular germ cells.
TEX101, a glycoprotein essential for sperm fertility, is required for stable expression of Ly6k on testicular germ cells.
复制标题
DOI：
10.1038/srep23616
复制
发表时间：
2016-03-23
影响因子：
4.6
通讯作者：
Araki Y
中科院分区：
文献类型：
--
作者：
Endo S;Yoshitake H;Tsukamoto H;Matsuura H;Kato K;Sakuraba M;Takamori K;Fujiwara H;Takeda S;Araki Y
TEX101是一种生殖细胞特异性糖基磷脂酰肌醇(GPI)锚定糖蛋白，与Ly6k在睾丸精子发生过程中相关。虽然Tex101−/−和Ly6k−/−小鼠都能产生形态完整的精子，但由于精子迁移到输卵管中的障碍，这两种基因敲除小鼠都表现出不育的表型。由于Ly6k与TEX101特异地相互作用，TEX101/Ly6k的复合体的形成似乎对功能性精子的产生具有潜在的重要意义。这项研究评估了Ly6k在TEX101存在或不存在的情况下的命运，以探索两种GPI锚定蛋白在生精小管中的分子相互作用。本研究表明：1)虽然在Tex101-−/−小鼠成熟睾丸中检测到Ly6kmRNA的表达，但表达水平很低；(2)在Tex101-−/−小鼠中Ly6kmRNA的表达在正常范围内；(3)在Tex101+/+和Tex101-−/−小鼠的睾丸中，Ly6kmRNA被翻译成多肽；这些结果表明，TEX101和Ly6k都参与了翻译后细胞膜上对应蛋白的表达。这一机制可能在精子发生过程中维持可育精子的产生方面起重要作用。
TEX101, a germ cell-specific glycosyl-phosphatidylinositol (GPI)-anchored glycoprotein, is associated with Ly6k during spermatogenesis in testis. Although both Tex101−/− and Ly6k−/− mice can produce morphologically intact spermatozoa, both knockout mice show an infertile phenotype due to a disorder of spermatozoa to migrate into the oviduct. Since Ly6k specifically interacts with TEX101, complex formation of TEX101/Ly6k appears to be potentially important for functional sperm production. This study evaluated the fate of Ly6k in the presence or absence of TEX101 to explore the molecular interaction of both GPI-anchored proteins in seminiferous tubules. The present study showed that: 1) Although Ly6k mRNA was detected, the protein was present at very low levels in mature testes of Tex101−/− mice, 2) Ly6k mRNA level was within the normal range in Tex101−/− mice, 3) Ly6k mRNA was translated into a polypeptide in the testes of Tex101+/+ and Tex101−/− mice, and 4) TEX101, as well as Ly6k, are co-factors that affect to molecular expression. These results indicate that both TEX101 and Ly6k contribute to the post-translational counterpart protein expression at the cell membrane. This mechanism may be important in maintaining the production of fertile spermatozoa during spermatogenesis.