14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.
14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.
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14-3-3epsilon 是一种核基质蛋白，其表达和定位的改变与姜黄素诱导的 MG-63 细胞凋亡有关。
DOI：
10.3892/ol.2017.7283
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发表时间：
2018-01
期刊：
影响因子：
2.9
通讯作者：
Li Q
中科院分区：
文献类型：
--
作者：
Lu K;Rui G;Liu F;Yang L;Deng X;Shi S;Li Q
14-3-3蛋白家族可能参与调节蛋白质相互作用、转运和细胞定位。14-3-3ε的调节作用受到其改变的定位的影响。在本研究中，姜黄素处理人骨肉瘤MG-63细胞诱导凋亡。随后，研究了14-3-3ε在细胞凋亡过程中的表达和定位变化及其与其他凋亡相关蛋白的共定位。应用双向凝胶电泳结合基质辅助激光解吸电离飞行时间质谱分析核基质蛋白（NMPs），发现14-3-3ε存在于MG-63细胞核基质上，姜黄素处理后其表达较对照组降低。Western blot分析证实，姜黄素诱导MG-63细胞凋亡过程中14-3-3ε的表达水平较对照组下调。用免疫荧光标记法观察到14-3-3ε在MG-63细胞核基质上的分布，姜黄素处理后14-3-3ε在MG-63细胞核基质上的分布较对照组减少。免疫荧光双标及激光共聚焦显微镜观察显示14-3-3ε与B细胞淋巴瘤2（B-cell lymphoma-2，Bcl-2-associated-X protein）、p53及c-FOS转录因子在MG-63细胞中共定位。此外，在用姜黄素处理后，这些共定位区域减少。本研究结果表明，14-3-3ε是MG-63细胞中的一种NMP，其表达的改变以及与凋亡相关蛋白的共定位表明14-3-3ε在MG-63细胞凋亡中起重要作用。需要进行更多的研究来调查本研究的结果。
The 14-3-3 protein family may regulates protein interaction, transportation and cellular localization. The regulatory role of 14-3-3ε is influenced by its altered localization. In the present study, human osteosarcoma MG-63 cells were treated with curcumin to induce apoptosis. Subsequently, the altered expression and localization of 14-3-3ε and its co-localization with other apoptosis-associated proteins during apoptosis was investigated. Analysis of nuclear matrix proteins (NMPs), using two-dimensional gel electrophoresis with matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry, revealed that 14-3-3ε existed on the nuclear matrix of MG-63 cells, and its expression was decreased compared with that in control cells following curcumin treatment. In addition, western blot analysis validated that the expression level of 14-3-3ε was downregulated during curcumin-induced apoptosis of MG-63 cells compared with that in control cells. Using immunofluorescence labeling, it was observed that 14-3-3ε was located on the nuclear matrix of MG-63 cells and the distribution of 14-3-3ε on the nuclear matrix was decreased following treatment with curcumin, compared with that in control cells. Double immunofluorescence staining and laser-scanning confocal microscopy demonstrated that 14-3-3ε was co-localized with B-cell lymphoma-2 (Bcl-2), Bcl-2-associated-X protein, p53 and c-FOS transcription factor in MG-63 cells. Furthermore, following treatment with curcumin, these co-localization regions were decreased. The results of the present study revealed that 14-3-3ε is an NMP in MG-63 cells, and its altered expression and co-localization with apoptosis-associated proteins indicated an important function of 14-3-3ε in apoptosis of MG-63 cells. Additional studies are required to investigate the results of the present study.
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影响因子：
14.9
作者：
NCBI Resource Coordinators
通讯作者：
NCBI Resource Coordinators
影响因子：
6.4
作者：
Cimino, Daniela;Fuso, Luca;De Bortoli, Michele
通讯作者：
De Bortoli, Michele
DOI：
10.1073/pnas.94.8.3668
发表时间：
1997-04-15
影响因子：
11.1
作者：
Hsu, YT;Wolter, KG;Youle, RJ
通讯作者：
Youle, RJ
影响因子：
5.3
作者：
Kosaka, Yasuhiro;Cieslik, Katarzyna A.;Brunelli, Luca
通讯作者：
Brunelli, Luca
影响因子：
5.1
作者：
Nagappan, Arulkumar;Park, Hyeon Soo;Kim, Gon Sup
通讯作者：
Kim, Gon Sup