Circulating microRNA-21 as a novel biomarker for hepatocellular carcinoma
Circulating microRNA-21 as a novel biomarker for hepatocellular carcinoma
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DOI：
10.1016/j.jhep.2011.04.026
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发表时间：
2012-01-01
影响因子：
25.7
通讯作者：
Mori, Masaki
中科院分区：
文献类型：
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作者：
Tomimaru, Yoshito;Eguchi, Hidetoshi;Mori, Masaki
背景与目的：一些研究小组已经报道了循环microRNA作为癌症生化标志物的重要性。然而，据我们所知，目前还没有关于循环microRNA在肝细胞癌中的意义的报道。本研究旨在探讨血浆microRNA-21水平作为肝细胞癌生化标志物的意义。方法：采用qRT-PCR方法检测10例肝细胞癌患者手术前后血浆microRNA-21水平。血浆microRNA-21也在其他组中进行了比较：126名肝细胞癌患者，30名慢性肝炎患者和50名健康志愿者。microRNA-21在区分肝细胞癌从慢性肝炎或从健康志愿者的权力进行了比较，甲胎蛋白。结果：在10例患者组，血浆microRNA-21水平显着减少手术后相比，术前值（p = 0.0125）。126例肝细胞癌患者血浆microRNA-21水平显著高于慢性肝炎患者和健康志愿者（p
Background & Aims: Several groups have reported the significance of circulating microRNA as a biochemical marker of cancer. To our knowledge, however, there are no reports on the significance of circulating microRNA in hepatocellular carcinoma. The aim of this study was to evaluate the significance of plasma microRNA-21 level as a biochemical marker for hepatocellular carcinoma.Methods: Plasma microRNA-21 level was measured by qRT-PCR in 10 patients before and after curative resection of hepatocellular carcinoma. Plasma microRNA-21 was also compared in other groups of: 126 patients with hepatocellular carcinoma, 30 patients with chronic hepatitis, and 50 healthy volunteers. The power of microRNA-21 in differentiating hepatocellular carcinoma from chronic hepatitis or from healthy volunteers was compared to that of a-fetoprotein.Results: In the 10-patient group, plasma microRNA-21 levels significantly diminished after surgery compared with the pre-operative values (p = 0.0125). Plasma microRNA-21 level in the 126 patients with hepatocellular carcinoma was significantly higher than in patients with chronic hepatitis and healthy volunteers (p