Confirmation of hepatitis C virus transmission through needlestick accidents by molecular evolutionary analysis.
Confirmation of hepatitis C virus transmission through needlestick accidents by molecular evolutionary analysis.
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通过分子进化分析证实丙型肝炎病毒通过针刺事故传播。
DOI：
10.1093/infdis/170.6.1575
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
T. Gojobori
中科院分区：
文献类型：
--
作者：
Kaoru Suzuki;M. Mizokami;Johnson Y.N. Lau;N. Mizoguchi;Koji Kato;Yoshiki Mizuno;T. Sodeyama;K. Kiyosawa;T. Gojobori
为了解丙型肝炎病毒（HCV）通过针刺事故传播的情况，采用基于HCV E2区的分子进化分析方法对3名因针刺事故感染丙型肝炎病毒的卫生工作者及其供体患者进行了研究。每个受试者至少有6个克隆被测序。采用六参数法估计核苷酸取代，采用邻域连接法构建系统发育树。来自供体患者和受体的HCV分离株被嵌套在一个单系群中；这种聚类通过bootstrap分析证实具有统计学显著性。来自受体的分离株之间的核苷酸差异始终小于来自供体的分离株，这支持了从供体到受体的传播方向的概念。这些发现在分子进化水平上提供了证据，证明丙型肝炎病毒是通过针刺事故传播的。
To document the transmission of hepatitis C virus (HCV) through needlestick accidents, 3 health workers who acquired HCV through such accidents and their HCV donor patients were studied using molecular evolutionary analysis based on the HCV E2 region. At least six clones were sequenced from each subject. Nucleotide substitutions were estimated by the six-parameter method, and a phylogenetic tree was constructed by the neighbor-joining method. HCV isolates from the donor patient and the recipient were nested in one monophyletic cluster; this clustering was confirmed to be statistically significant by bootstrap analysis. The nucleotide divergence among the isolates from the recipient was always smaller than that from the donor, supporting the notion that the direction of transmission was from the donor to the recipient. These findings provide evidence, at a molecular evolutionary level, that HCV was transmitted through needlestick accidents.
宿主细胞基因衍生表位 GOR 抗体在慢性丙型肝炎病毒感染中的意义。
影响因子：
25.7
作者：
Lau,JY;Davis,GL;Orito,E;Qian,KP;Mizokami,M
通讯作者：
Mizokami,M