Morphological phenotypes of olfactory ensheathing cells display different migratory responses upon Slit-2.
Morphological phenotypes of olfactory ensheathing cells display different migratory responses upon Slit-2.
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DOI：
10.1016/j.yexcr.2012.05.024
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发表时间：
2012-09
影响因子：
3.7
通讯作者：
Ying Wang;Zhihui Huang
中科院分区：
文献类型：
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作者：
Ying Wang;Zhihui Huang
嗅鞘细胞（Olfactory ensheathing cells，OECs）是嗅觉系统中一类具有形态可塑性的神经胶质细胞。培养的嗅鞘细胞呈扁平状。我们前期的研究表明，在锋面施加Slit-2梯度，可以诱导前缘的崩溃，逆转含过程嗅鞘细胞的索马移位。然而，平坦的嗅鞘细胞在Slit-2梯度上的迁移特性仍然难以捉摸。在这里，我们发现Slit-2梯度诱导其质膜的崩溃，并抑制扁平OECs的迁移。在Slit-2梯度下，扁平1型嗅鞘细胞的前缘首先出现塌陷和回缩，然后逐渐重新长出新的片状伪足，最后再次出现塌陷（这种现象称为适应），而扁平2型嗅鞘细胞仅出现质膜塌陷。这些不同的迁移反应可能是由于不同的亚细胞分布的Robo受体的Slit-2刺激。此外，F-actin在前缘的周边区域比微管对Slit-2刺激更敏感，并且F-actin的丢失可能与启动扁平OEC的崩溃有关。最后，由Slit-2诱导的扁平1型嗅鞘细胞的适应性不依赖于蛋白质合成。综上所述，这些结果表明，OECs的形态表型显示不同的迁移特性后，Slit-2和一个意想不到的发现，蛋白质合成独立的适应OECs诱导的Slit-2。
Olfactory ensheathing cells (OECs) are a type of glial cells with morphological plasticity in the olfactory system. Cultured OECs display the process-bearing and flattened shape. Our previous studies have shown that the frontal application of Slit-2 gradient induced the collapse of leading front, and reversed the soma translocation of process-bearing OECs. However, the migratory properties of flattened OECs upon Slit-2 gradient remain elusive. Here, we found that Slit-2 gradient induced the collapse of their plasma membrane, and inhibited migration of flattened OECs. Upon to Slit-2 gradient, the leading front of flattened type 1 OECs firstly showed collapse and retraction, then gradually re-grew a new lamellipodia, finally, showed collapse again (this phenomenon was called as adaptation), while flattened type 2 OECs only showed collapse of plasma membrane. These different migratory responses upon Slit-2 stimulation were possibly due to their different sub-cellular distribution of Robo receptor. Furthermore, F-actin at the peripheral region of leading front was more sensitive to the Slit-2 stimulation than microtubules and the loss of F-actin might be implicated in initiating the collapse of flattened OECs. Finally, the adaptation of flattened type 1 OECs induced by Slit-2 was independent on protein synthesis. Taken together, these results demonstrate that morphological phenotypes of OECs display different migratory properties upon Slit-2 and an unexpected finding that the protein synthesis-independent adaptation in OECs induced by Slit-2.