Production of cellulase-free xylanase by a thermotolerant Streptomyces sp grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods
Production of cellulase-free xylanase by a thermotolerant Streptomyces sp grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods
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DOI：
10.1023/a:1019827321162
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发表时间：
2002-09-01
影响因子：
2.7
通讯作者：
Poosaran, N
中科院分区：
文献类型：
--
作者：
Techapun, C;Sinsuwongwat, S;Poosaran, N
链霉菌Ab 106在55 ℃下利用甘蔗渣产生无纤维素酶木聚糖酶。采用混料设计和线性数学规划确定了最佳培养基组成，并采用Plackett-Burman实验设计进行了评价。采用混料设计法确定了最佳培养基组成。在摇瓶发酵条件下，100 rpm，55 ℃，pH 7，发酵6 d后，木聚糖酶活力最高，为10.6 IU。两个实验设计均表明微量元素诱导木聚糖酶的产生。在5升发酵罐中发酵，木聚糖酶活性达到12.5 IU。
Cellulase-free xylanase was produced by Streptomyces sp. Ab106 on finely ground cane bagasse at 55 degreesC. The optimal medium composition was developed by applying the mixture design and linear mathematical program, and evaluated using the Plackett-Burman experimental design. The best composition of basal medium was found by using the mixture design method. The highest xylanase activity, 10.6 IU, was obtained after 6 days of fermentation in shaked flask at 100 rpm, 55 degreesC, pH 7. Both experimental designs showed that trace elements induced xylanase production. With fermentation in a 5-l fermenter, xylanase activity of 12.5 IU was achieved.