The biophysical mechanisms of altered hyaluronan concentration in synovial fluid after anterior cruciate ligament transection.
The biophysical mechanisms of altered hyaluronan concentration in synovial fluid after anterior cruciate ligament transection.
复制标题
前交叉韧带横断后滑液中透明质酸浓度改变的生物物理机制。
DOI：
10.1002/art.37682
复制
发表时间：
2012
影响因子：
--
通讯作者：
Sah,RobertL
中科院分区：
文献类型：
--
作者：
McCarty,WilliamJ;Cheng,JustinC;Hansen,BradleyC;Yamaguchi,Tomonori;Firestein,GaryS;Masuda,Koichi;Sah,RobertL
目的采用兔前交叉韧带横断(ACLT)模型，研究透明质酸(HA)在膝关节滑液中的滞留时间。本研究的目的是评估术后7天和28天时，兔膝关节滑膜和滑膜细胞的HA浓度和分子质量(MR)分布，2)生理盐水冲洗后HA的内源性补充，3)HA在SF中的停留时间，4)膝关节滑膜和滑膜下细胞的数量，并与假手术和非手术对照进行比较。术后第7天或第28天，抽吸关节液，生理盐水灌洗，注射生理盐水或多分散羟基磷灰石，于注射后8h取材进行分析。分析关节液中HA的浓度和MR分布，以计算HA的滞留时间常数。结果HA浓度和MR分布的分析显示：1)在第7天，高MRHA在SF中丢失，并在第28天向低MR分布转变；2)洗脱后高MRHA的内源性补充；以及3)膝关节HA依赖MR的丢失，尤其是在术后第7天。HA的停留时间随HAMR的减少而减少(停留时间∼为27小时，MR负荷为7,000-2,500kd，∼为7小时，MR负荷为250-50 kd)。在ACLT后第7天，HA在膝关节中的停留时间也减少了(∼减少了70%)。结论HA在关节滑膜中的滞留时间一过性缩短，提示关节损伤后或炎症过程中滑膜成分的改变可能与生物物理转运机制有关。
ObjectiveThe residence time of hyaluronan (HA) in knee joint synovial fluid (SF) was investigated using a rabbit anterior cruciate ligament transection (ACLT) model. The aims of this study were to assess, at 7 and 28 days after surgery, the 1) HA concentration and molecular mass (Mr) distribution in the SF, 2) endogenous replenishment of HA after saline washout, 3) HA residence times in the SF, and 4) synovium and subsynovium cellularity of the knee joints of rabbits subjected to ACLT, compared to sham‐operated and nonoperated control joints.MethodsAdult NZW rabbits underwent ACLT or sham surgery on one hind limb, while each contralateral limb was the nonoperated control. On day 7 or 28 after surgery, the joints were aspirated for SF, lavaged with saline, and injected with saline or polydisperse HA, and samples were obtained for analysis at set time points up to 8 hours after injection. Joint fluid samples were analyzed for the concentration andMrdistribution of HA to calculate the HA residence time constant.ResultsAnalysis of HA concentrations andMrdistributions showed 1) loss of high‐MrHA in the SF on day 7 and a shift toward a lower‐Mrdistribution on day 28, 2) endogenous replenishment of high‐MrHA after washout, and 3)Mr‐dependent loss of HA from the knee joints after ACLT, particularly on day 7 postsurgery. The HA residence time decreased with decreasing HAMr(residence time ∼27 hours with anMrload of 7,000–2,500 kd, to ∼7 hours with anMrload of 250–50 kd). HA residence time also decreased (by ∼70%) in the knee joints on day 7 after ACLT. The subsynovium of the joints subjected to ACLT displayed increased cellularity and neovascularization on days 7 and 28 postsurgery.ConclusionThe residence time of HA in the SF is transiently decreased after ACLT, suggesting that a biophysical transport mechanism is responsible for the altered composition of the SF after joint injury or during inflammation.