Sequence Analysis and Domain Motifs in the Porcine Skin Decorin Glycosaminoglycan Chain*
Sequence Analysis and Domain Motifs in the Porcine Skin Decorin Glycosaminoglycan Chain*
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DOI：
10.1074/jbc.m112.437236
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发表时间：
2013-02
期刊：
影响因子：
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通讯作者：
Xue Zhao;Bo Yang;Kemal Solakylidirim;E. Joo;T. Toida;Kyohei Higashi;R. Linhardt;Lingyun Li
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文献类型：
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作者：
Xue Zhao;Bo Yang;Kemal Solakylidirim;E. Joo;T. Toida;Kyohei Higashi;R. Linhardt;Lingyun Li
背景：核心蛋白聚糖的糖基化修饰是一种重要的蛋白质，但其结构基序和序列尚不清楚。结果：FT-MS已定位结构域，并测序了一条小的GAG链。结论：GAG链结构因来源而异，但基序结构似乎相对一致，可能由少量序列组成。意义：了解GAG的结构可能有助于了解其生物合成和生物学功能。核心蛋白聚糖蛋白聚糖由含有单个0-连接的硫酸皮肤素/硫酸软骨素糖胺聚糖（GAG）链的核心蛋白组成。虽然核心蛋白聚糖的序列是由编码其结构的基因决定的，但其GAG链的结构是由高尔基体决定的。最近将现代MS应用于bikunin，一种更简单的硫酸软骨素蛋白聚糖，表明它具有单个或少量的定义序列。在此基础上，采用类似的方法对猪皮肤的核心蛋白聚糖进行测序，所述核心蛋白聚糖更大且结构更复杂的硫酸皮肤素/硫酸软骨素GAG链。该方法产生了关于其在GAG链还原端的连接区、其富艾杜糖醛酸结构域、富葡萄糖醛酸结构域和非还原端的一致性/可变性的信息。建立了猪皮核心蛋白聚糖GAG链的通用基序。使用MS/MS分析对单个小核心蛋白聚糖GAG链进行测序。研究中获得的数据表明核心蛋白聚糖GAG链具有少量或有限数量的序列。
Background: GAG of decorin is important, but its structural motifs and sequences are unknown. Results: FT-MS has mapped domains, and a small GAG chain was sequenced. Conclusion: GAG chain structure varies depending on origin, but motif structure appears relatively consistent and may be comprised of a small number of sequences. Significance: Understanding GAG structure may lead to understanding its biosynthesis and biological functions. Decorin proteoglycan is comprised of a core protein containing a single O-linked dermatan sulfate/chondroitin sulfate glycosaminoglycan (GAG) chain. Although the sequence of the decorin core protein is determined by the gene encoding its structure, the structure of its GAG chain is determined in the Golgi. The recent application of modern MS to bikunin, a far simpler chondroitin sulfate proteoglycans, suggests that it has a single or small number of defined sequences. On this basis, a similar approach to sequence the decorin of porcine skin much larger and more structurally complex dermatan sulfate/chondroitin sulfate GAG chain was undertaken. This approach resulted in information on the consistency/variability of its linkage region at the reducing end of the GAG chain, its iduronic acid-rich domain, glucuronic acid-rich domain, and non-reducing end. A general motif for the porcine skin decorin GAG chain was established. A single small decorin GAG chain was sequenced using MS/MS analysis. The data obtained in the study suggest that the decorin GAG chain has a small or a limited number of sequences.