Dissecting Phagosomal Pattern Recognition Receptor-Dependent Signaling and Antigen MHC-II Presentation from Phagosomes in Murine Dendritic Cells.
Dissecting Phagosomal Pattern Recognition Receptor-Dependent Signaling and Antigen MHC-II Presentation from Phagosomes in Murine Dendritic Cells.
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剖析小鼠树突状细胞中吞噬体的吞噬体模式识别受体依赖性信号传导和抗原 MHC-II 呈递。
DOI：
10.1007/978-1-0716-3338-0_19
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Mantegazza,AdrianaR
中科院分区：
文献类型：
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作者：
Scharrig,Emilia;Mantegazza,AdrianaR
吞噬体模式识别受体信号传导促进吞噬体成熟和其他免疫途径，如促炎细胞因子分泌和抗原呈递细胞中的抗原MHC-II呈递。在本章中，我们描述了在小鼠树突状细胞中评估这些途径的程序，树突状细胞是位于先天免疫反应和适应性免疫反应之间的专业吞噬细胞。本文描述的实验是在通过生化和免疫学检测促炎信号传导以及通过免疫荧光和流式细胞术检测模型抗原Eα的抗原呈递之后进行的。
Phagosomal pattern recognition receptor signaling promotes phagosome maturation and additional immune pathways such as proinflammatory cytokine secretion and antigen MHC-II presentation in antigen-presenting cells. In the present chapter, we describe procedures to assess these pathways in murine dendritic cells, professional phagocytes positioned at the interface between innate and adaptive immune responses. The assays described herein follow proinflammatory signaling by biochemical and immunological assays as well as antigen presentation of the model antigen Eα by immunofluorescence followed by flow cytometry.