Extractive Spectrophotometric Determination of Fluoroquinolones and Antiallergic Drugs in Pure and Pharmaceutical Formulations
Extractive Spectrophotometric Determination of Fluoroquinolones and Antiallergic Drugs in Pure and Pharmaceutical Formulations
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DOI：
10.2116/analsci.19.461
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发表时间：
2003-03
影响因子：
1.6
通讯作者：
B. G. Gowda;J. Seetharamappa
中科院分区：
文献类型：
--
作者：
B. G. Gowda;J. Seetharamappa
本文提出了一种简便、快速、灵敏的分光光度法测定氟喹诺酮类药物的含量，环丙沙星（CPF）和诺氟沙星（NRF），以及抗过敏药物，盐酸甲地拉嗪（MDH）和盐酸异噻苯啶（IPH）的原料药和药物制剂。方法是在pH4.0的NaOAc-AcOH缓冲溶液中，CPF和NRF与亮蓝G（BBG）反应，MDH和IPH与BBG反应，生成可溶于氯仿的离子缔合物。研究了各种参数的影响。CPF、NRF和MDH、IPH的最大吸收波长分别为610 nm、614 nm和612 nm。CPF、NRF、MDH和IPH的浓度分别在0.5 - 6.0、0.4 - 8.0、0.1-6.0和0.2 - 6.1（mg/ml）范围内符合比尔定律，相关系数不小于0.9969。根据比尔定律数据计算出的CPF、NRF、MDH和IPH的摩尔吸光系数值分别为2.86 × 10^4、2.64 × 10^4、3.13 × 10^4和5.51 × 10^4 l mol^-1 cm^-1。研究了常用赋形剂对药物测定的影响。所提出的方法得到的结果进行了统计验证。
A simple, rapid and sensitive spectrophotometric procedure has been proposed for the assay of fluoroquinolones viz ., ciprofloxacin (CPF) and norfloxacin (NRF), and antiallergic drugs viz ., methdilazine hydrochloride (MDH) and isothipendyl hydrochloride (IPH) in bulk and pharmaceutical formulations. The method is based on the reaction of selected drugs with Brilliant Blue G (BBG) in NaOAc-AcOH buffer of pH 4.0 for CPF and NRF or in neutral medium for MDH and IPH to give chloroform soluble ion-association complexes. The effects of various parameters have been studied. The ion-association complexes exhibited absorption maxima at 610 nm for CPF, at 614 nm for NRF and MDH, and at 612 nm for IPH. Beer’s law plots were obeyed in the concentration ranges of 0.5 - 6.0, 0.4 - 8.0, 0.1–6.0 and 0.2 - 6.1 (mg ml^-1 for CPF, NRF, MDH and IPH, respectively, with correlation coefficients not less than 0.9969. Molar absorptivity values as calculated from the Beer’s law data were found to be 2.86 × 10^4, 2.64 × 10^4, 3.13 × 10^4 and 5.51 × 10^4 l mol^-1 cm^-1 for CPF, NRF, MDH and IPH, respectively. The influence of commonly employed excipients in the determination of the studied drugs has been studied. The results obtained by the proposed method were statistically validated.