Expression profiles of genes involved in fatty acid and triacylglycerol synthesis in developing seeds of Jatropha (Jatropha curcas L.)
Expression profiles of genes involved in fatty acid and triacylglycerol synthesis in developing seeds of Jatropha (Jatropha curcas L.)
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麻风树种子发育过程中脂肪酸和三酰甘油合成相关基因的表达谱
DOI：
10.1016/j.biombioe.2011.01.001
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发表时间：
2011-05-01
影响因子：
6
通讯作者：
Liu, Aizhong
中科院分区：
文献类型：
--
作者：
Xu, Ronghua;Wang, Ruling;Liu, Aizhong
麻风树（Jatropha curcas L.，大戟科植物是一种潜在的生物柴油植物，其种子油作为生物柴油的商业来源在世界各地引起了极大的兴趣。麻风树种子含油量不可靠，经济效益低，限制了麻风树的农业种植。研究种子发育过程中贮藏脂肪积累的分子基础是了解麻风树种子贮藏脂肪生物合成调控的遗传因素的迫切需要。在这项研究中，我们的特点是种子发育和脂质积累从雌花授粉成熟种子，并调查了21个脂质基因的时间表达谱参与的途径的不同步骤，导致脂肪酸和TAG的合成在麻风树发展中的种子使用定量实时PCR技术。与此同时，17个基因在发育种子中的表达水平比在叶片中的表达水平增加，但在发育种子中表现出不同的时间表达模式和不同的相对最大比率，范围从2.8到1，919，280倍。通过表达数据的聚类分析确定了具有不同时间模式的五个基因组。两组共15个基因在发育中的种子中呈现与贮藏脂肪积累相关的上调表达模式。这项研究不仅提供了每个基因启动子活性的初步信息，而且还首次看到了基因表达和调控的全球模式，这对于了解脂质生物合成的分子基础，确定种子发育过程中的限速基因以及通过基因工程创造改良品种至关重要。(C)2011爱思唯尔有限公司版权所有。
Jatropha (Jatropha curcas L., Euphorbiaceae), a potential biodiesel plant, has created tremendous interest all over the world for the use of its seed oil as a commercial source of biodiesel. Due to the unreliability of oil content in its seeds and low economic returns planting of jatropha in agriculture was restricted. Investigating the molecular basis of storage lipid accumulation during seed development is an immediate need to understand genetic factors regulating storage lipid biosynthesis in jatropha seeds. In this study, we characterized the seed development and lipid accumulation from female flowers pollinated to mature seeds, and investigated temporal expression profiles of 21 lipid genes involved in different steps of the pathways leading to fatty acid and TAG synthesis within jatropha developing seeds using quantitative real-time PCR technology. Concomitantly, 17 genes increased their expression levels in developing seeds compared to their expression in leaf, but showed various temporal expression patterns and different relative-maximum ratio ranging from 2.8 to 1,919,280-fold in developing seeds. Five gene groups with distinct temporal patterns were identified by clustering analysis of expression data. Two gene groups including 15 genes presented up-regulated expression patterns correlated with storage lipid accumulation in developing seeds. This study provided not only the initial information on promoter activity for each gene, but also a first glimpse of the global patterns of gene expression and regulation, which are critical to understand the molecular basis of lipid biosyntheses, identifying the rate-limiting genes during seed development and to create improved varieties by genetic engineering. (C) 2011 Elsevier Ltd. All rights reserved.