The characterization and manipulation of the bacterial microbiome of the Rocky Mountain wood tick, Dermacentor andersoni.
The characterization and manipulation of the bacterial microbiome of the Rocky Mountain wood tick, Dermacentor andersoni.
复制标题
DOI：
10.1186/s13071-015-1245-z
复制
发表时间：
2015-12-10
影响因子：
3.2
通讯作者：
Brayton KA
中科院分区：
文献类型：
--
作者：
Clayton KA;Gall CA;Mason KL;Scoles GA;Brayton KA
在北美，蜱是人类和动物病原体的最具经济影响力的载体。落基山木蜱，Dermacentor andersoni（阿卡里：硬蜱科），分别向人类和牛传播立克次体和边缘无浆体。近年来的研究表明，共生生物参与了许多生化和生理功能。对D. andersoni是理解共生体-宿主相互作用的关键一步。在这项研究中，我们已经通过高通量序列分析表明，组成的内共生体在中肠和唾液腺在成年蜱是动态的三代。四变形菌属，立克次体，弗朗西斯，砷，和不动杆菌，被确定为这两个组织中的优势共生菌。暴露于治疗剂量的广谱抗生素，土霉素，影响两个主要属的比例，并显着降低生殖健康。此外，不动杆菌是一种自由生活的普遍存在的微生物，基于抗生素治疗状态以不同比例侵入细菌微生物组，表明微生物组组成可能在对环境污染物的易感性中起作用。本研究对D. andersoni，并确定了该蜱内的世代变异性。此外，这项研究证实了微生物组操纵与蜱适应性有关，可能是一种潜在的生物防治方法。
In North America, ticks are the most economically impactful vectors of human and animal pathogens. The Rocky Mountain wood tick, Dermacentor andersoni (Acari: Ixodidae), transmits Rickettsia rickettsii and Anaplasma marginale to humans and cattle, respectively. In recent years, studies have shown that symbiotic organisms are involved in a number of biochemical and physiological functions. Characterizing the bacterial microbiome of D. andersoni is a pivotal step towards understanding symbiont-host interactions. In this study, we have shown by high-throughput sequence analysis that the composition of endosymbionts in the midgut and salivary glands in adult ticks is dynamic over three generations. Four Proteobacteria genera, Rickettsia, Francisella, Arsenophonus, and Acinetobacter, were identified as predominant symbionts in these two tissues. Exposure to therapeutic doses of the broad-spectrum antibiotic, oxytetracycline, affected both proportions of predominant genera and significantly reduced reproductive fitness. Additionally, Acinetobacter, a free-living ubiquitous microbe, invaded the bacterial microbiome at different proportions based on antibiotic treatment status suggesting that microbiome composition may have a role in susceptibility to environmental contaminants. This study characterized the bacterial microbiome in D. andersoni and determined the generational variability within this tick. Furthermore, this study confirmed that microbiome manipulation is associated with tick fitness and may be a potential method for biocontrol.