Therapeutic effects of hybrid liposomes on mice model of lung carcinoma.
Therapeutic effects of hybrid liposomes on mice model of lung carcinoma.
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混合脂质体对小鼠肺癌模型的治疗作用。
DOI：
10.2745/dds.14.37
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发表时间：
1999
影响因子：
--
通讯作者：
R. Ueoka
中科院分区：
文献类型：
--
作者：
A. Kanno;Yasutaka Terada;K. Tsuzaki;Y. Matsumoto;R. Ueoka
我们以前开发了一种新的医用材料杂化脂质体。杂化脂质体可以简单地通过对含有磷脂和胶束表面活性剂的水性缓冲溶液施加超声处理来制备;它们不受任何有机溶剂的污染，并且与常规脂质体相比更长时间稳定。我们已经证明了杂交脂质体在体外对淋巴瘤细胞具有抗肿瘤作用。此外，含有脂溶性抗肿瘤剂的复合脂质体能显著延长胶质瘤小鼠模型的生存时间。本研究制备了由90 mol% L-α-二肉豆蔻酰磷脂酰胆碱（DMPC）和10 mol%聚氧乙烯（10）十二烷基醚（C12（EO）10）组成的均匀稳定的混合脂质体，其流体动力学直径为80 nm。DMPC/10 mol% C12（EO）10（[DMPC]=0.383 mM）的混合脂质体对体外培养的刘易斯肺癌细胞的50 mol%抑制浓度小于DMPC脂质体（>1 mM）。腹腔接种刘易斯肺癌细胞建立小鼠肺癌模型。对照组小鼠肺癌模型的存活时间中位数和最大值分别为25.0和45.0天。另一方面，DMPC/10 mol% C12（EO）10混合脂质体处理组的存活时间超过1年。这些结果表明，杂化脂质体对肺癌的治疗是有效的。
We have previously developed a new medical material of hybrid liposomes. The hybrid liposomes can be prepared simply by applying sonication to the aqueous buffer solution which contains phospholipids and micellar surfactants ; they are free from any contamination of organic solvent, and more stable for a long time as compared with conventional liposomes. We have already demonstrated that the hybrid liposomes have an antitumor effect on lymphoma cells in vitro. Furthermore, the hybrid liposomes including lipid-soluble antitumor agent were found to markedly prolong the life span of mice model of gliorna. In this study, the uniform and stable hybrid liposomes composed of 90 mol% L-α-dimyristoylphosphatidylcholine (DMPC) and 10 mol% polyoxyethylene(10)dodecyl ether (C12(EO)10) having a hydrodynamic diameter of 80 nm were prepared for the therapeutical application. The 50 mol% inhibitory concentration of the hybrid liposomes of DMPC/10 mol% C12(EO)10, ([DMPC]=0.383 mM) on the growth of Lewis lung carcinoma cells in vitro was smaller than that of DMPC liposomes (>1 mM). A mouse model of lung carcinoma was established by intraperitoneal inoculation of Lewis lung carcinoma cells. The survival times of mice model of lung carcinoma in the control groups were 25.0 and 45.0 days for median and maximum values, respectively. On the other hand, the survival times in the treated groups with the hybrid liposomes of DMPC/10 mol% C12(EO)10 were more than one year. These results suggest that the hybrid liposomes should be effective for the treatment of lung carcinoma.