In vivo receptor binding: attempts to improve specific/non-specific ratios.
In vivo receptor binding: attempts to improve specific/non-specific ratios.
复制标题
体内受体结合：尝试改善特异性/非特异性比率。
DOI：
10.1016/0024-3205(79)90539-3
复制
发表时间：
1979
期刊：
影响因子：
6.1
通讯作者：
M. Kuhar
中科院分区：
文献类型：
--
作者：
D. Niehoff;J. Palacios;M. Kuhar
使用3 H-螺哌隆和3 H-匹莫齐特检测多巴胺受体的体内受体结合，使用3 H-LSD检测血清素受体的体内受体结合。两种策略，提高总：非特异性结合率进行了测试。第一种是在3 H-配体给药前通过各种药物治疗耗尽内源性配体，以增加特异性受体结合;第二种是在3 H-配体给药后用冰冷盐水灌注脑，以减少非特异性结合。多巴胺和5-羟色胺的改变，管理d-苯丙胺，利血平，α-甲基-对酪氨酸或对氯苯丙氨酸并没有显着提高纹状体：小脑或皮质：小脑（措施总：非特异性）的结合率。然而，用冰冷的盐水灌注显著改善了多巴胺和5-羟色胺受体的比率。因此，冷生理盐水灌注可能在降低放射自显影和其他需要体内标记受体的研究中的空白值方面具有价值。
In vivo receptor binding was examined using 3 H-spiperone and 3 H-pimozide for dopamine receptors and 3 H-LSD for serotonin receptors. Two strategies for improving total: nonspecific binding ratios were tested. The first was to deplete endogenous ligands by various pharmacological treatments prior to 3 H-ligand administration in an attempt to increase specific receptor binding; the second was to perfuse the brain with ice-cold saline after 3 H-ligand administration in an attempt to reduce nonspecific binding. Alteration of dopamine and serotonin by administering d-amphetamine, reserpine, alpha-methyl-paratyrosine or parachlorophenylalanine did not significantly elevate striatal: cerebellar or cortical: cerebellar (measures of total: nonspecific) bonding ratios. However, perfusion with ice-cold saline significantly improved the ratios for both dopamine and serotonin receptors. Thus, cold saline perfusion may be of value in reducing blank values in autoradiographic and other studies requiring in vivo labelling of receptors.