In vitro susceptibility tests for cationic peptides: Comparison of broth microdilution methods for bacteria that grow aerobically
In vitro susceptibility tests for cationic peptides: Comparison of broth microdilution methods for bacteria that grow aerobically
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DOI：
10.1128/aac.44.6.1694-1696.2000
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发表时间：
2000-06-01
影响因子：
4.9
通讯作者：
Scalise, G
中科院分区：
文献类型：
--
作者：
Giacometti, A;Cirioni, O;Scalise, G
采用两种肉汤微量稀释法，对90株革兰氏阳性和革兰氏阴性需氧菌对6种阳离子肽buforin II、天蚕素P1、indolicidin、magainin II、nisin和ranalexin的体外敏感性进行了评价。第一种方法是根据国家临床实验室标准委员会概述的需氧细菌生长程序进行的，而第二种方法是根据R. E. W.汉考克实验室测试抗菌肽。总的来说，第一种方法产生的MIC比第二种方法高出两到四倍。
The in vitro susceptibilities of 90 clinical isolates of gram-positive and gram-negative aerobic bacteria to six cationic peptides, buforin II, cecropin P1, indolicidin, magainin II, nisin, and ranalexin, were evaluated by two broth microdilution methods. The first method was performed according to the procedures outlined by the National Committee for Clinical Laboratory Standards for bacteria that grow aerobically, while the second was performed according to the procedures recently proposed by the R. E. W. Hancock laboratory for testing antimicrobial peptides. Overall, the first method produced MICs two- and fourfold higher than the second method.